Changes in phenylalanine ammonia-lyase activity and gene expression during storage of asparagus spears

被引：0
|
作者：
Bhowmik, PK [1 ]
Matsui, T
机构：
[1] Natl Agr Res Ctr, Sapporo, Hokkaido 0628555, Japan
[2] Kagawa Univ, Fac Agr, Dept Bioresource Prod Sci, Kagawa 7610795, Japan
来源：
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES | 2005年 / 60卷 / 1-2期
关键词：
asparagus spear; gene expression; phenylalanine ammonia-lyase;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A cDNA clone coding phenylalanine ammonia-lyase (PAL) was isolated from a cDNA library prepared from asparagus spears (Asparagus officinalis L. cv. Welcome) using the reverse transcription-polymerase chain reaction (RT-PCR). The partial cDNA clone encoded an mRNA of 527 bp and the derived amino acid sequence was found highly homologous to PAL from rice, maize and barley. Northern blot analysis showed an increase of pAS-PAL mRNA until 24 h at 20 degrees C, which coincided well with PAL activity and fiber development, suggesting that the increase is a response to the wounding associated with harvest.
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页码：128 / 132
页数：5
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