Evaluation of a PCR targeting fimbrial subunit gene (fimA) for rapid and reliable detection of Enteroaggregative Escherichia coli recovered from human and animal diarrhoeal cases

被引：3

作者：

Rawool, Deepak B. ^{[1
]}

Vergis, Jess ^{[1
]}

Vijay, Deepthi ^{[1
]}

Dhaka, Pankaj ^{[1
]}

Negi, Mamta ^{[1
]}

Kumar, Manesh ^{[1
]}

Nair, Amruta ^{[1
]}

Poharkar, Krupali V. ^{[3
]}

Kurkure, Nitin V. ^{[3
]}

Kumar, Ashok ^{[1
]}

Malik, S. V. S. ^{[1
]}

Barbuddhe, S. B. ^{[2
]}

机构：

[1] Indian Vet Res Inst, Div Vet Publ Hlth, Izatnagar 243122, Uttar Pradesh, India

[2] Natl Inst Biot Stress Management, Raipur 492012, Chhattisgarh, India

[3] Nagpur Vet Coll, MAFSU, Dept Vet Pathol, Nagpur 440006, Maharashtra, India

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2015年 / 110卷

关键词：

Diarrhoea; EAEC; fimA; HEp-2; PCR; VIRULENCE; STRAINS; CHILDREN; EPIDEMIOLOGY; BRAZIL; CELLS;

D O I：

10.1016/j.mimet.2015.01.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study describes the utility of a chromosomal associated fimbrial subunit gene (fimA) for screening 'typical' as well as 'atypical' Enteroaggregative Escherichia coli (EAEC) by PCR and its possible role as a promising molecular marker for rapid detection of 'typical' and 'atypical' EAEC pathotype. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：45 / 48

页数：4

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