An affinity-based probe for the proteomic profiling of aspartic proteases

被引：23

作者：

Chattopadhaya, S

Chan, EWS

Yao, SQ

机构：

[1] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

[2] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

来源：

TETRAHEDRON LETTERS | 2005年 / 46卷 / 23期

关键词：

affinity-based profiling; activity-based profiling; proteomics; aspartic proteases; enzymes; photolabile probe;

D O I：

10.1016/j.tetlet.2005.04.015

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We have developed an affinity-based probe for the proteomic profiling of aspartic proteases. Our probe was shown to be selective towards aspartic proteases over other proteins. It was also shown that the strategy may be used to label selectively aspartic proteases in the presence of a large excess of other proteins, thus making it useful for future proteome profiling experiments. (c) 2005 Elsevier Ltd. All rights reserved.

引用

收藏

页码：4053 / 4056

页数：4

相关论文

共 17 条

[1] Proteomic profiling of mechanistically distinct enzyme classes using a common chemotype [J].

Adam, GC ;

Sorensen, EJ ;

Cravatt, BF .

NATURE BIOTECHNOLOGY, 2002, 20 (08) :805-809

[2] Mass spectrometry-based proteomics [J].

Aebersold, R ;

Mann, M .

NATURE, 2003, 422 (6928) :198-207

[3]

BARRETT AJ, 2004, HDB PROTEOLYTIC ENZY

[4] Developing photoactive affinity probes for proteomic profiling: Hydroxamate-based probes for metalloproteases [J].

Chan, EWS ;

Chattopadhaya, S ;

Panicker, RC ;

Huang, X ;

Yao, SQ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (44) :14435-14446

[5] Affinity-based tagging of protein families with reversible inhibitors: A concept for functional proteomics [J].

Hagenstein, MC ;

Mussgnug, JH ;

Lotte, K ;

Plessow, R ;

Brockhinke, A ;

Kruse, O ;

Sewald, N .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (45) :5635-5638

[6] The development and application of methods for activity-based protein profiling [J].

Jessani, N ;

Cravatt, BF .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2004, 8 (01) :54-59

[7]

LIN YZ, 1992, J BIOL CHEM, V267, P18413

[8] Activity-based protein profiling: The serine hydrolases [J].

Liu, YS ;

Patricelli, MP ;

Cravatt, BF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (26) :14694-14699

[9] Design and synthesis of class-selective activity probes for protein tyrosine phosphatases [J].

Lo, LC ;

Pang, TL ;

Kuo, CH ;

Chiang, YL ;

Wang, HY ;

Lin, JJ .

JOURNAL OF PROTEOME RESEARCH, 2002, 1 (01) :35-40

[10]

MARCINISZYN J, 1976, J BIOL CHEM, V251, P7095