Assessment of the TGFB1 gene expression and methylation status of the promoter region in patients with colorectal cancer

被引：6

作者：

Wodzinski, Damian ^{[1
]}

Wosiak, Agnieszka ^{[1
]}

Pietrzak, Jacek ^{[1
]}

Swiechowski, Rafal ^{[1
]}

Kordek, Radzislaw ^{[2
]}

Balcerczak, Ewa ^{[1
]}

机构：

[1] Med Univ Lodz, Interfac Cathedral Lab & Mol Diagnost, Dept Pharmaceut Biochem & Mol Diagnost, Lab Mol Diagnost & Pharmacogen, Lodz, Poland

[2] Med Univ Lodz, Dept Pathol, Cathedral Oncol, Lodz, Poland

来源：

SCIENTIFIC REPORTS | 2022年 / 12卷 / 01期

关键词：

MESENCHYMAL-TRANSITION; SIGNALING PATHWAY; BREAST-CANCER; BETA; TGF-BETA-1; MIGRATION; INVASION; PROLIFERATION; INHIBITION; ACTIVATION;

D O I：

10.1038/s41598-022-15599-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of this study was to evaluate the expression of the TGFB1 gene encoding the TGF-beta 1 cytokine in 64 patients, and then to compare it with clinico-pathological features. The study also investigated whether the regulation of the gene expression is caused by methylation of the promoter region between - 235 and + 22 nucleotide from the start of transcription. The dependence of the relative level of the TGFB1 gene expression on the clinical advancement according to the TNM classifications was shown. Additionally, the individual grades of the T and M features of the TNM classification differed in the relative transcript levels of the TGFB1 gene. Moreover, the higher relative expression level of the studied gene was associated with a lack of vascular invasion by cancer cells and presence of lymphocytes in the neoplastic tissue. The obtained results may indicate a possible impact of the gene on the process of carcinogenesis in colorectal cancer and reduction of its expression level may be one of the factors contributing to progression of the disease.

引用

页数：12