New cationic liposomes as vehicles of m-tetrahydroxyphenylchlorin in photodynamic therapy of infectious diseases

被引：85

作者：

Bombelli, Cecilia ^{[1
]}

Bordi, Federico ^{[2
,3
]}

Ferro, Stefania ^{[4
]}

Giansanti, Luisa ^{[5
]}

Jori, Giulio ^{[4
]}

Mancini, Giovanna ^{[1
]}

Mazzuca, Claudia ^{[6
]}

Monti, Donato ^{[6
]}

Ricchelli, Fernanda ^{[4
]}

Sennato, Simona ^{[2
,3
]}

Venanzi, Mariano ^{[6
]}

机构：

[1] Ist Metodol Chim, CNR, I-00185 Rome, Italy

[2] Univ Roma La Sapienza, Dipartimento Fis, I-00185 Rome, Italy

[3] Univ Roma La Sapienza, INFM CRS SOFT, I-00185 Rome, Italy

[4] Univ Padua, Dipartimento Biol, CNR Ist Tecnol Biomed, Sez Metalloprot, I-35121 Padua, Italy

[5] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

[6] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, I-00133 Rome, Italy

来源：

MOLECULAR PHARMACEUTICS | 2008年 / 5卷 / 04期

关键词：

methicillin-resistant bacteria; cationic liposomes; photodynamic therapy; bactericidal activity;

D O I：

10.1021/mp800037d

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Antimicrobial photodynamic therapy is emerging as a promising therapeutic modality for bacterial infections. For optimizing the antibacterial activity of the photosensitizer in-tetrahydroxyphenylchlorin, it has been encapsulated in mixed cationic liposomes composed of different ratios of dimyristoyl-sn-glycero-phosphatidylcholine and any of four cationic surfactants derived from L-prolinol. The delivery efficiency of the different liposomes formulations has been Evaluated on a methicillin-resistant Staphylococcus aureus bacterial strain (MRSA), and one of the tested formulations shows a biological activity comparable to that of the free chlorin. In order to rationalize the physicochemical parameters of the carriers that control the biological activity, the new liposome formulations have been characterized by measuring (a) the zeta potential, (b) the r capability of chlorin entrapping efficiency, i.e. entrapment efficacy, (c) the effect of storage on chlorin entrapment and (d) the localization of chlorin in the bilayer. The correlation of the physicochemical and biological features of formulations has allowed us to rationalize, to some extent, some of the parameters that may control the interactions with the biological environment.

引用

页码：672 / 679

页数：8

共 21 条

[1]

[Anonymous], 1996, FUNDAMENTAL BASES PH

[2] Efficient transfection of DNA by liposomes formulated with cationic gemini amphiphiles [J].