Pulsed-field gel electrophoresis for the discrimination of Oenococcus oeni isolates from different wine-growing regions in Germany

被引：31

作者：

Larlsika, Melanie ^{[1
]}

Claus, Harald ^{[1
]}

Koenig, Helmut ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Inst Microbiol & Wine Res, D-55099 Mainz, Germany

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2008年 / 123卷 / 1-2期

关键词：

Oenococcus oeni; rotating-field gel electrophoresis; strain differentiation; cluster analysis;

D O I：

10.1016/j.ijfoodmicro.2007.11.081

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Reliable techniques are needed for the identification individual Oenococeus oeni strains with desirable flavor characteristics and to monitor the survival and contribution of inoculated and indigenous bacteria. Therefore, we investigated the Suitability Of pulsed-field gel electrophoresis (PFGE) for the discrimination of 65 O. oeni isolates from six different wine-producing regions in Germany. Among the restriction enzymes tested, genomic DNA digestions with Sfi I were most effective by displaying 56 (86%) different banding profiles. Our results underline the high capacity of PFGE for strain identification and differentiation. Cluster analysis of the DNA restriction patterns revealed no distinct region specificity of O. oeni Populations. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：171 / 176

页数：6

共 29 条

[1]

CLAUS H, 1995, MICROBIOL RES, V150, P159, DOI 10.1016/S0944-5013(11)80051-0

[2] OPTIMIZATION OF TRANSVERSE ALTERNATING-FIELD ELECTROPHORESIS FOR STRAIN IDENTIFICATION OF LEUCONOSTOC-OENOS [J].

DANIEL, P ;

DEWAELE, E ;

HALLET, JN .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1993, 38 (05) :638-641

[3] Allelic diversity and population structure in Oenococcus oeni as determined from sequence analysis of housekeeping genes [J].

de las Rivas, B ;

Marcobal, A ;

Muñoz, R .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (12) :7210-7219

[4] Intraspecific diversity of Oenococcus oeni strains determined by sequence analysis of target genes [J].

Delaherche, Arnaud ;

Bon, Elisabeth ;

Dupe, Aurelien ;

Lucas, Megumi ;

Arveiler, Benoit ;

De Daruvar, Antoine ;

Lonvaud-Funel, Aline .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 73 (02) :394-403

[5] TAXONOMY OF LEUCONOSTOC SPECIES, PARTICULARLY LEUCONOSTOC-OENOS, AS REVEALED BY NUMERICAL-ANALYSIS OF TOTAL SOLUBLE CELL PROTEIN-PATTERNS, DNA-BASE COMPOSITIONS, AND DNA-DNA HYBRIDIZATIONS [J].

DICKS, LMT ;

VANVUUREN, HJJ ;

DELLAGLIO, F .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1990, 40 (01) :83-91

[6]

DICKS LMT, 1990, FEMS MICROBIOL LETT, V67, P9, DOI 10.1016/S0378-1097(98)00376-0

[7]

DICKS LTM, 1995, INT J SYSTEMATIC BAC, V45, P295

[8] Oenococcus kitaharae sp nov., a non-acidophilic and non-malolactic-fermenting oenococcus isolated from a composting distilled shochu residue [J].

Endo, Akihito ;

Okada, Sanae .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2006, 56 :2345-2348

[9]

Fugelsang KC., 2007, WIN MICR PRACT APPL, V2nd

[10] Phenotypic and genotypic characterization of Oenococcus oeni strains isolated from Italian wines [J].

Guerrini, S ;

Bastianini, A ;

Blaiotta, G ;

Granchi, L ;

Moschetti, G ;

Coppola, S ;

Romano, P ;

Vincenzini, M .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2003, 83 (01) :1-14

← 1 2 3 →