Topographic mapping of VMH → arcuate nucleus microcircuits and their reorganization by fasting

被引:246
作者
Sternson, SM
Shepherd, GMG
Friedman, JM
机构
[1] Rockefeller Univ, Howard Hughes Med Inst, New York, NY 10021 USA
[2] Rockefeller Univ, Mol Genet Lab, New York, NY 10021 USA
[3] Howard Hughes Med Inst, Cold Spring Harbor, NY 11724 USA
[4] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1038/nn1550
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In the hypothalamic arcuate nucleus ( ARC), pro-opiomelanocortin ( POMC) neurons inhibit feeding and neuropeptide-Y (NPY) neurons stimulate feeding. We tested whether neurons in the ventromedial hypothalamic nucleus (VMH), a known satiety center, activate anorexigenic neuronal pathways in the ARC by projecting either excitatory synaptic inputs to POMC neurons and/or inhibitory inputs to NPY neurons. Using laser scanning photostimulation in brain slices from transgenic mice, we found that POMC and NPY neurons, which are interspersed in the ARC, are nevertheless regulated by anatomically distinct synaptic inputs. POMC neurons received strong excitatory input from the medial VMH (mVMH), whereas NPY neurons did not and, instead, received weak inhibitory input only from within the ARC. The strength of the excitatory input from the mVMH to POMC neurons was diminished by fasting. These data identify a new molecularly defined circuit that is dynamically regulated by nutritional state in a manner consistent with the known role of the VMH as a satiety center.
引用
收藏
页码:1356 / 1363
页数:8
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