Graphene oxide coated Titanium Surfaces with Osteoimmunomodulatory Role to Enhance Osteogenesis

被引:58
作者
Su, Jiehua [1 ,2 ,5 ]
Du, Zhibin [5 ,6 ]
Xiao, Lan [5 ,6 ]
Wei, Fei [5 ,6 ]
Yang, Ying [5 ,6 ]
Li, Mengting [7 ]
Qiu, Yubei [3 ,4 ]
Liu, Jiali [1 ,2 ]
Chen, Jiang [1 ,2 ,5 ]
Xiao, Yin [6 ]
机构
[1] Fujian Med Univ, Sch & Hosp Stomatol, Inst Stomatol, Fuzhou, Peoples R China
[2] Fujian Med Univ, Sch & Hosp Stomatol, Lab Oral Tissue Engn, Fuzhou, Peoples R China
[3] Fujian Med Univ, Fujian Key Lab Oral Dis, Oral Biomat & Stomatol Key Lab, Fujian Coll & Univ,Sch & Hosp Stomatol, Fuzhou, Peoples R China
[4] Fujian Med Univ, Fujian Prov Engn Res Ctr, Oral Biomat & Stomatol Key Lab, Fujian Coll & Univ,Sch & Hosp Stomatol, Fuzhou, Peoples R China
[5] Queensland Univ Technol, Inst Hlth & Biomed Innovat, 60 Musk Ave, Brisbane, Qld 4059, Australia
[6] Queensland Univ Technol, Australia China Ctr Tissue Engn & Regenerat Med A, Brisbane, Qld, Australia
[7] Hainan Univ, Hainan Prov Fine Chem Engn Res Ctr, Haikou 570228, Hainan, Peoples R China
来源
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2020年 / 113卷
基金
中国国家自然科学基金;
关键词
Graphene Oxide; Titanium; Osteoblasts; Macrophages; Cell differentiation; Implants; MESENCHYMAL STEM-CELLS; OSTEOBLAST DIFFERENTIATION; BONE SIALOPROTEIN; MARROW; INDUCTION; FRACTURE; BIOMATERIALS; REGENERATION; MACROPHAGES; RECRUITMENT;
D O I
10.1016/j.msec.2020.110983
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Graphene oxide (GO) and its derivatives are currently being explored for the modification of bone biomaterials. However, the effect of GO coatings on immunoregulation and subsequent impacts on osteogenesis are not known. In this study, GO was coated on pure titanium using dopamine. GO-coated titanium (Ti-GO) surfaces exhibited good biocompatibility, with the ability to stimulate the expression of osteogenic genes, and extracellular matrix mineralization in human mesenchymal stromal cells (hMSCs). Interestingly, it was found that GO-coated surfaces could manipulate the polarization of macrophages and expression of inflammatory cytokines via the Toll-like receptor pathway. Under physiological conditions, Ti-GO activated macrophages and induced mild inflammation and a pro-osteogenic environment, characterized by a slight increase in the levels of proinflammatory cytokines, as well as increased expression of the TGF-beta 1 and oncostatin M genes. In an environment mimicking acute inflammatory conditions, Ti-GO attenuated inflammatory responses, as shown by the downregulation of proinflammatory cytokines. Conditioned medium collected from macrophages stimulated by Ti-GO played a significant stimulatory role in the osteogenic differentiation of hMSCs. In summary, GO-coated surfaces displayed beneficial immunomodulatory effects in osteogenesis, indicating that GO could be a potential substance for the modification of bone scaffolds and implants.
引用
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页数:14
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