Circular RNA expression profiles and bioinformatic analysis in coronary heart disease

被引：25

作者：

Yu, Fangpu ^{[1
,2
,3
]}

Tie, Yuanyuan ^{[1
,2
,3
]}

Zhang, Ya ^{[1
,2
,3
]}

Wang, Zunzhe ^{[1
,2
,3
]}

Yu, Liwen ^{[1
,2
,3
]}

Zhong, Lin ^{[4
]}

Zhang, Cheng ^{[1
,2
,3
]}

机构：

[1] Shandong Univ, Chinese Minist Hlth, Key Lab Cardiovasc Remodeling & Funct Res, Chinese Minist Educ, Jinan 250012, Shandong, Peoples R China

[2] Shandong Univ, Chinese Acad Med Sci, Jinan 250012, Shandong, Peoples R China

[3] Shandong Univ, State & Shandong Prov Joint Key Lab Translat Card, Qilu Hosp, Jinan 250012, Shandong, Peoples R China

[4] Qingdao Univ, Yuhuangding Hosp, Cardiol Dept, Sch Med, Yantai 264000, Shandong, Peoples R China

来源：

EPIGENOMICS | 2020年 / 12卷 / 05期

基金：

中国国家自然科学基金;

关键词：

circular RNAs (circRNAs); coronary heart disease (CHD); miRNA; RNA sequencing (RNA-seq); ATHEROSCLEROSIS; PROLIFERATION; CELLS;

D O I：

10.2217/epi-2019-0369

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Aim: We aimed to identify the expression profile and role of circular RNAs (circRNAs) in coronary heart disease (CHD). Materials & methods: We performed sequence analysis of circRNAs in peripheral blood mononuclear cells of 70 CHD patients and 30 controls. Eight selected circRNAs were validated using quantitative real-time polymerase chain reaction (qRT-PCR) in human atherosclerotic coronary arteries. Results: In total, 2283 downregulated and 85 upregulated circRNAs were identified in CHD. Parental genes of top 100 dysregulated-circRNAs are related to metabolism and protein modification, and 12 circRNAs might upregulate their CHD-related parental genes through miRNA sponges. Of the eight circRNAs validated in atherosclerotic coronary arteries by qRT-PCR, six were consistent with sequencing results of peripheral blood mononuclear cells. Conclusion: As potential ceRNAs, dysregulated circRNAs may be involved in CHD pathophysiology.

引用

页码：439 / 454

页数：16

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