Intra-specific diversity of Aureobasidium pullulans strains isolated from rocks and other habitats assessed by physiological methods and by random amplified polymorphic DNA (RAPD)

被引：49

作者：

Urzì, C ^{[1
]}

De Leo, F ^{[1
]}

Lo Passo, C ^{[1
]}

Criseo, G ^{[1
]}

机构：

[1] Univ Messina, Fac Sci, Inst Microbiol, I-98166 Messina, Italy

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1999年 / 36卷 / 1-2期

关键词：

A-pillulans; RAPD; rock colonisation;

D O I：

10.1016/S0167-7012(99)00014-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Intra-specific diversity of Aureobasidium pullulans strains isolated from environmental sources and from stones was studied by assessment of morphological, biochemical and physiological characters as well as random amplified polymorphic DNA (RAPD) using microsatellite or minisatellite DNA primers (GTG)(5), (GACA)(4), M13. The results showed that both classical and molecular techniques evidenced a phenotypic and genetic diversity of analysed A. pullulans strains. A different behaviour was observed in reference to the growth responses with D-glucosamine, citrate, galactitol and with different salt concentrations and range of growth temperature. Molecular analysis partially confirmed the data obtained with biochemical and physiological tests, additionally showing common fragments in all strains, to be used for a possible application as 'in situ' probes and for a rapid identification of A. pullulans strains. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：95 / 105

页数：11

共 22 条

[1]

AJELLO L, 1977, PAHO, V324, P9

[2]

[Anonymous], 1977, Stud. Mycol

[3]

Arnaud G, 1910, ANN MYCOL, V8, P470

[4]

BRAAMS J, 1993, THESIS OLDENBURG

[5] Classification and identification of bacteria: Current approaches to an old problem. Overview of methods used in bacterial systematics [J].

Busse, HJ ;

Denner, EBM ;

Lubitz, W .

JOURNAL OF BIOTECHNOLOGY, 1996, 47 (01) :3-38

[6]

De Leo F, 1996, P 8 INT C DET CONS S, P625

[7]

DEBARY, 1866, MORPHOL PHYSL PILZE, P182

[8] EVOLUTION OF BLACK YEASTS - POSSIBLE ADAPTATION TO THE HUMAN HOST [J].

DEHOOG, GS .

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 1993, 63 (02) :105-109

[9] CONIDIOGENESIS, NUTRITIONAL PHYSIOLOGY AND TAXONOMY OF AUREOBASIDIUM AND HORMONEMA [J].

DEHOOG, GS ;

YURLOVA, NA .

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 1994, 65 (01) :41-54

[10]

DEHOOG GS, 1987, EXPANDING REALM YEAS, P187

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