Immobilization of laccase on magnetic bimodal mesoporous carbon and the application in the removal of phenolic compounds

被引：230

作者：

Liu, Yuanyuan ^{[1
,2
]}

Zeng, Zhuotong ^{[5
]}

Zeng, Guangming ^{[1
,2
]}

Tang, Lin ^{[1
,2
]}

Pang, Ya ^{[1
,2
]}

Li, Zhen ^{[1
,2
]}

Liu, Can ^{[1
,2
]}

Lei, Xiaoxia ^{[1
,2
]}

Wu, Mengshi ^{[1
,2
]}

Ren, Pinyun ^{[3
,4
]}

Liu, Zhifeng ^{[1
,2
]}

Chen, Ming ^{[1
,2
]}

Xie, Gengxin ^{[1
,2
]}

机构：

[1] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China

[2] Hunan Univ, Minist Educ, Key Lab Environm Biol & Pollut Control, Changsha 410082, Hunan, Peoples R China

[3] Hunan Univ, Coll Phys & Microelect Sci, Changsha 410082, Hunan, Peoples R China

[4] Hunan Univ, Key Lab Micronano Phys & Technol Hunan Prov, Changsha 410082, Hunan, Peoples R China

[5] Cent S Univ, State Key Lab Med Genet, Changsha 410078, Hunan, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 115卷

基金：

中国国家自然科学基金;

关键词：

Immobilization; Laccase; Magnetic mesoporous carbon; Phenol removal; REVERSIBLE IMMOBILIZATION; TRAMETES-VERSICOLOR; AQUEOUS-SOLUTIONS; ADSORPTION; FABRICATION; OXIDATION; DEGRADATION;

D O I：

10.1016/j.biortech.2011.11.015

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A novel magnetically separable laccase immobilized system was constructed by adsorbing laccase into bimodal carbon-based mesoporous magnetic composites (CMMC). A large adsorption capacity (491.7 mg g(-1)), excellent activity recovery (91.0%) and broader pH and temperature profiles than free laccase have been exhibited by the immobilized laccase. Thermal stability was enhanced to a great extent and operational stability was increased to a certain extent. The shift of kinetic parameters indicated affinity change between enzyme and substrate. Application of the immobilized system in phenol and p-chlorophenol removal was investigated in a batch system. Adsorption effects of the support were responsible for the quick removal rate in the first hour, and up to 78% and 84% of phenol and p-chlorophenol were removed in the end of the reaction, respectively, indicating that the magnetic bimodal mesoporous carbon is a promising carrier for both immobilization of laccase and further application in phenol removal. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.

引用

页码：21 / 26

页数：6

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