Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp (MSI057)

被引：65

作者：

Kiran, G. Seghal ^{[1
,2
]}

Shanmughapriya, S. ^{[1
]}

Jayalakshmi, J. ^{[1
]}

Selvin, Joseph ^{[1
]}

Gandhimathi, R. ^{[1
]}

Sivaramakrishnan, S. ^{[1
,2
]}

Arunkumar, M. ^{[1
]}

Thangavelu, T. ^{[1
]}

Natarajaseenivasan, K. ^{[1
]}

机构：

[1] Bharathidasan Univ, Dept Microbiol, Tiruchirappalli 620024, Tamil Nadu, India

[2] Bharathidasan Univ, Dept Biotechnol, Tiruchirappalli 620024, Tamil Nadu, India

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2008年 / 31卷 / 05期

关键词：

extracellular lipase; sponge endosymbionts; Pseudomonas; optimization; psychrophilic lipase;

D O I：

10.1007/s00449-007-0186-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An endosymbiotic Pseudomonas sp. (MSI057), which could produce high yields of lipase, was isolated from marine sponge Dendrilla nigra, collected from the peninsular coast of India. Maximum production of enzyme was obtained in minimal medium supplemented with 1% tributyrin. Catabolite repression was observed when the medium was supplemented with readily available carbon sources. The optimum temperature and pH for the enzyme production was 30 degrees C and 9.0, respectively. The enzyme exhibited maximum activity in pH range of 8-9 with an optimum pH 9.0. The activity of purified enzyme was optimum at 37 degrees C and showed 80% activity at 20 degrees C and the enzyme activity decreased dramatically above 50 degrees C. Based on the present findings, the enzyme was characterized as psychrophilic alkaline lipase, which can be developed for industrial applications.

引用

页码：483 / 492

页数：10

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