Advanced glycation end products alter the m6A-modified RNA profiles in human dermal fibroblasts

被引：6

作者：

Ouyang, Mengting ^{[1
]}

Fang, Jiaqi ^{[1
]}

Wang, Mengyao ^{[1
]}

Huang, Xianyin ^{[1
]}

Lan, Jingjing ^{[1
]}

Qu, Yingying ^{[1
]}

Lai, Wei ^{[1
]}

Xu, Qingfang ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Dept Dermatovenereol, Affiliated Hosp 3, Guangzhou 510630, Peoples R China

来源：

EPIGENOMICS | 2022年 / 14卷 / 08期

基金：

中国国家自然科学基金;

关键词：

advanced glycation end products; bioinformatics analysis; fibroblasts; lncRNA; m(6)A methylation; photoaging; skin; NF-KAPPA-B; MESSENGER-RNA; DOWN-REGULATION; EXPRESSION; ACTIVATION; M(6)A; INFLAMMATION; RECEPTOR; PATHWAY; METHYLATION;

D O I：

10.2217/epi-2022-0016

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Background: To explore advanced glycation end products (AGEs)-induced m(6)A modification in fibroblasts and its potential role in photoaging. Methods: We studied m(6)A modification in AGEs-bovine serum albumin-treated fibroblasts with m(6)A-mRNA & lncRNA epitranscriptomic microarray and bioinformatics analysis. The m(6)A modification level was also investigated in skin samples. Results: m(6)A methylation microarray analysis revealed m(6)A modification profiles in AGEs-treated fibroblasts. Gene ontology, Kyoto Encyclopedia of Genes and Genomes, protein-protein interaction and competing endogenous RNA network analysis indicated that the genes of differentially methylated mRNAs and lncRNAs were mainly related to inflammation processes. We also found that AGEs-bovine serum albumin dose-dependently increased the m(6)A level and METTL14 expression in both fibroblasts and sun-exposed skin. Conclusion: Our study provided novel information regarding alterations of m(6)A modifications in AGEs-induced dermal fibroblasts and potential targets for treatment of photoaging.

引用

页码：431 / 449

页数：19

共 49 条

[1] Context-dependent Cooperation between Nuclear Factor κB (NF-κB) and the Glucocorticoid Receptor at a TNFAIP3 Intronic Enhancer [J].

Altonsy, Mohammed O. ;

Sasse, Sarah K. ;

Phang, Tzu L. ;

Gerber, Anthony N. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (12) :8231-8239

[2] AGEs and Glucose Levels Modulate Type I and III Procollagen mRNA Synthesis in Dermal Fibroblasts Cells Culture [J].

Andreea, Serban Iren ;

Marieta, Costache ;

Anca, Dinischiotu .

EXPERIMENTAL DIABETES RESEARCH, 2008, :473603

[3] A20 down-regulation promotes UVB-induced inflammation via activation of the NF-κB pathway [J].

Chen, Yan ;

Wu, Wenjuan ;

Li, Xing ;

Guo, Yanni ;

Lu, Fengyan ;

Man, Maoqiang ;

Jin, Yumei ;

Yang, Chunyan ;

Chai, Yanjie ;

He, Li .

JOURNAL OF DERMATOLOGICAL SCIENCE, 2020, 98 (01) :61-64

[4] Expression of Advanced Glycation End-Products on Sun-Exposed and Non-Exposed Cutaneous Sites during the Ageing Process in Humans [J].

Crisan, Maria ;

Taulescu, Marian ;

Crisan, Diana ;

Cosgarea, Rodica ;

Parvu, Alina ;

Catoi, Cornel ;

Drugan, Tudor .

PLOS ONE, 2013, 8 (10)

[5] Advanced Glycation End Products (AGES) Induce Apoptosis of Fibroblasts by Activation of NLRP3 Inflammasome via Reactive Oxygen Species (ROS) Signaling Pathway [J].

Dai, Jiezhi ;

Chen, Hua ;

Chai, Yimin .

MEDICAL SCIENCE MONITOR, 2019, 25 :7499-7508

[6] Modulation of the pro-inflammatory cytokines and matrix metalloproteinases production in co-cultivated human keratinocytes and melanocytes [J].

Decean, H. ;

Perde-Schrepler, M. ;

Tatomir, C. ;

Fischer-Fodor, E. ;

Brie, I. ;

Virag, P. .

ARCHIVES OF DERMATOLOGICAL RESEARCH, 2013, 305 (08) :705-714

[7] Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq [J].

Dominissini, Dan ;

Moshitch-Moshkovitz, Sharon ;

Schwartz, Schraga ;

Salmon-Divon, Mali ;

Ungar, Lior ;

Osenberg, Sivan ;

Cesarkas, Karen ;

Jacob-Hirsch, Jasmine ;

Amariglio, Ninette ;

Kupiec, Martin ;

Sorek, Rotem ;

Rechavi, Gideon .

NATURE, 2012, 485 (7397) :201-U84

[8] Interplay Between N6-Methyladenosine (m6A) and Non-coding RNAs in Cell Development and Cancer [J].

Fazi, Francesco ;

Fatica, Alessandro .

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2019, 7

[9] Gene expression regulation mediated through reversible m6A RNA methylation [J].

Fu, Ye ;

Dominissini, Dan ;

Rechavi, Gideon ;

He, Chuan .

NATURE REVIEWS GENETICS, 2014, 15 (05) :293-306

[10] Dietary AGEs involvement in colonic inflammation and cancer: insights from an in vitro enterocyte model [J].

Geicu, Ovidiu I. ;

Stanca, Loredana ;

Voicu, Sorina N. ;

Dinischiotu, Anca ;

Bilteanu, Liviu ;

Serban, Andreea I. ;

Calu, Valentin .

SCIENTIFIC REPORTS, 2020, 10 (01)

← 1 2 3 4 5 →