The impact of ovarian stimulation with recombinant FSH in combination with GnRH antagonist on the endometrial transcriptome in the window of implantation

被引：56

作者：

Macklon, Nick S. ^{[1
]}

van der Gaast, Mark H. ^{[2
]}

Hamilton, Amy ^{[3
]}

Fauser, Bart C. J. M. ^{[1
]}

Gludice, Linda C. ^{[3
]}

机构：

[1] UMC Utrecht, Dept Reprod Med & Gynaecol, NL-3584 CX Utrecht, Netherlands

[2] Erasmus MC, Dept Obstet & Gynecol, Rotterdam, Netherlands

[3] Univ Calif San Francisco, Dept Obstet & Gynecol, San Francisco, CA 94143 USA

来源：

REPRODUCTIVE SCIENCES | 2008年 / 15卷 / 04期

关键词：

endometrium; gene expression; ovarian stimulation;

D O I：

10.1177/1933719107311781

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The aim of this prospective paired cohort study is to elucidate the impact Of ovarian stimulation with recombinant-follicle-stimulating hormone in combination with gonadotropin-releasing hormone antagonist on the endometrial transcrimome. Oocyte donors undenvent endometrial biopsy during the implantation window of the nonstimulated cycle and following ovarian stimulation with recombinant follicle-stimulating hormone and gonadotropin-releasing hormone antagonist but no luteal progesterone supplementation (n = 4). Microarray analysis showed 142 genes to be significantly upregulated and 98 significantly downregulated. Significantly upregulated genes included those sequencing for the chemokine ligand CXCL 13, the Dickkopf homolog, steroidcgenic acute regulatory protein, and homeobox C6. Also upregulated were genes inhibited by progesterone, such as insulin-like growth factor binding protein 5. In conclusion, ovarian stimulation with follicle-stimulating hormone and gonadotropin-releasing hormone antagonist dysregulates the expression of many genes involved in cell adhesion, T-cell receptor signaling, and regulation of signal transduction. These data suggest that dysregulation of the endometrial transcriptome in the stimulated cycle is not fully attributable to supraphysiological sex steroid levels at the folliculo-luteal transition.

引用

页码：357 / 365

页数：9

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