Isolation and structural identification of a novel fructan from Radix Codonopsis

被引:11
|
作者
Li, Jiankuan [1 ]
Wang, Yan [1 ]
Zhang, Xia [1 ]
Cao, Lingya [1 ,2 ]
Ji, Jiaojiao [1 ]
Zheng, Qinghong [1 ]
Gao, Jianping [1 ]
机构
[1] Shanxi Med Univ, Sch Pharmaceut Sci, Taiyuan, Peoples R China
[2] Shanxi Med Univ, Sch Basic Med Sci, Taiyuan, Peoples R China
基金
国家重点研发计划;
关键词
Codonopsis pilosula; fructan; inulin; polysaccharide; INULIN-TYPE FRUCTANS; LEVAN; POLYSACCHARIDES;
D O I
10.1080/07328303.2020.1772278
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fructans are generally divided into two different types:beta-D-fructofuranosyl-(2 -> 1)-linked inulin and beta-D-fructofuranosyl-(2 -> 6)-linked levan. Previously, Radix Codonopsis was found to be rich in inulin type of fructans with low molecular weight (<5000 Da). In this study, a novel macromolecular fructan with a molecular weight of 1.70 x 10(3)KD was isolated from Radix Codonopsis, and its homogeneity was shown to be a single peak by high performance gel permeation chromatography (HPGPC), and its structure was confirmed to contain a new type of linkage,alpha-D-fructofuranosyl-(2 -> 3)-beta-D-fructofuranosyl linkage, by 1D- and 2D-nuclear magnetic resonance (NMR).
引用
收藏
页码:163 / 174
页数:12
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