Detection of dilute sperm samples using photoacoustic flowmetry

被引：0
作者：
Viator, J. A. [1 ]
Sutovsky, P. [1 ]
Weight, R. M. [2 ]
机构：
[1] Univ Missouri, Columbia, MO 65211 USA
[2] Kansas City Univ Med & Biosci, Kansas City, MO USA
来源：
PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2008: THE NINTH CONFERENCE ON BIOMEDICAL THERMOACOUSTICS, OPTOACOUSTICS, AND ACOUSTIC-OPTICS | 2008年 / 6856卷
关键词：
cytometry; flowmetry; optoacoustic;
D O I：
10.1117/12.764105
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Detection of sperm cells in dilute samples may have application in forensic testing and diagnosis of male reproductive health. Due to the optically dense subcellular structures in sperm cells, irradiation by nanosecond laser pulses induces a photoacoustic response detectable using a custom flow cytometer. We determined the detection threshold of bull sperm using various concentrations, from 200 to 1,000,000 sperm cells per milliliter. Using a tunable laser system set to 450nm with a 5ns pulse duration and 11-12 mJ/pulse, we obtained a detection threshold of 3 sperm cells. The flow rate was 4 ml/minute through the flow chamber. The acoustic sensor was a 100 mu m PVDF film attached to the glass flow chamber. The acoustic signal was preamplified and sent to an oscilloscope. The threshold signal indicated a signal to noise ratio of approximately 6 to 1. Improved system design may decrease the threshold to single sperm cells.
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页数：5
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