Should we use remifentanil in every dose and every case?

Aim: We widely use remifentanil as a pain killer in all cases that need surgery such as: broken bone, intestine, brain disease, organ transplantation. But we need to apply any of opioid analgesics according to the surgery type. The aim of the study is to determine neurotoxic or neuroprotective effects of different remifentanil doses in glutamate induced toxicity model in olfactory cell culture. Material and Method: Olfactory neurons were obtained from pupil of newborn Sprague dawley rat. Glutamate (10-5 mM) was added to all culture dishes except negative control group. Remifentanil was added in three different doses for 24 hours, and then evaluation was done by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Total Antioxidant Capacity (TAC), Total Oxidant Status (TOS) and Flow cytometry (Annexin V- apoptosis marker). Results: Our data shows the highest and the lowest viability obtained from low and high remifentanil dose around %91 and %75 respectively. TAC and TOS results have correlation with MTT results. TAC capacity in remifentanil 0,2 mM group shows nearest to control group value. Discussion: According to our result, remifentanil has potential to decrease toxicity level of glutamate and increases cell viability ratio.