Monoclonal Antibodies to Botulinum Neurotoxins of Types A, B, E, and F

被引：9
作者：
Abbasova, S. G. [1 ]
Rudenko, N. V. [1 ]
Gorokhovatskii, A. Yu. [1 ]
Kapralova, M. V. [2 ]
Vinogradova, I. D. [3 ]
Vertiev, Yu. V. [3 ]
Nesmeyanov, V. A. [4 ]
Grishin, E. V. [4 ]
机构：
[1] Russian Acad Sci, Pushchino Branch, Shemyakin Ovchinnikov Inst Bioorgan Chem, Pushchino 142290, Moscow Oblast, Russia
[2] Pushchino State Univ, Pushchino 142290, Moscow Oblast, Russia
[3] Russian Acad Med Sci, Gamalei Res Inst Microbiol & Epidemiol, Moscow, Russia
[4] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2011年 / 37卷 / 03期
关键词：
botulism; botulinum neurotoxins of types A; B; E; and F; monoclonal antibodies; production; sandwich ELISA; toxin neutralization; TOXIN; SEROTYPE; IDENTIFICATION; CLEAVAGE;
D O I：
10.1134/S1068162011030022
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mouse monoclonal antibodies to health-threatening botulinum neurotoxins (BoNTs) of types A, B, E, and F have been produced and characterized. The antibodies are capable of interacting with a toxin inside the respective natural toxic complex. A sandwich ELISA for the quantitative detection of botulotoxins has been developed on based on the antibodies. The detection limits of the test systems for BoNTs A, B, E, and F is 0.4, 0.5, 0.1, and 2.4 ng/ml, respectively. The assay quantitatively detects BoNTs in canned meat and vegetables. Two antibodies, BNTA-4.1 and BNTA-9.1, both separately and in combination, are capable of neutralizing the natural botulinum toxic complex of type A in vivo; a combination of antibodies neutralizes a higher dose of the toxin. It has been shown that the antibody BNTA-4.1 binds specifically to the light (catalytic) chain of the toxin, and the antibody BNTA-9.1 interacts with the heavy chain. We believe that monoclonal antibodies BNTA-4.1 and BNTA-9.1 hold promise for developing therapeutic antibodies to treat BoNT/A-caused botulism in an emergency.
引用
收藏
页码：307 / 315
页数：9
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