Label-free electronic detection of thrombin in blood serum by using an aptamer-based sensor

被引：678
作者：
Xiao, Y
Lubin, AA
Heeger, AJ
Plaxco, KW [1 ]
机构：
[1] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA
[2] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Santa Barbara, CA 93106 USA
[3] Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA
[4] Univ Calif Santa Barbara, Dept Mat, Santa Barbara, CA 93106 USA
[5] Univ Calif Santa Barbara, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 34期
关键词：
aptamers; biosensors; DNA structures; electron transfer; thrombin;
D O I：
10.1002/anie.200500989
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Blue, gold, and DNA: A methylene blue (MB) tagged, thrombin-binding DNA aptamer immobilized on a gold surface undergoes a large conformational change upon target binding (see schematic representation; eT: electron transfer). This folding produces a large, readily measurable change in redox current and allows the electrochemical detection of thrombin in blood serum. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.
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收藏
页码：5456 / 5459
页数：4
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