Early inflammatory profiling of schwannoma cells induced by lipopolysaccharide

被引:5
作者
Kohoutkova, Marcela [1 ]
Korimova, Andrea [1 ]
Brazda, Vaclav [1 ]
Kohoutek, Jiri [2 ]
Dubovy, Petr [1 ]
机构
[1] Masaryk Univ, Fac Med, Div Neuroanat, Dept Anat, Kamenice 3, Brno 62500, Czech Republic
[2] Masaryk Univ, RECETOX Res Ctr Tox Cpds Environm, Fac Sci, Brno, Czech Republic
关键词
Schwann cells; Toll-like receptor 4; Early endosomes; Cytokines; Transforming factors; FIBRILLARY ACIDIC PROTEIN; PERIPHERAL-NERVE; WALLERIAN DEGENERATION; SIGNALING PATHWAY; NEUROPATHIC PAIN; GENE-EXPRESSION; CYTOKINES; INTERLEUKIN-6; REGENERATION; INJURY;
D O I
10.1007/s00418-017-1601-5
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Inflammatory profiling of Schwann cells manifested as an upregulation of cytokines is present after traumatic or disease injury of the peripheral nerves. Inflammatory activation of Schwann cells via Toll-like receptors (TLRs) can be triggered by exogenous pathological molecules or endogenous ligands produced during Wallerian degeneration. We investigated the early period of inflammatory reactions by following the levels of TLR4, NF kappa B, IL-1 beta, pSTAT3, and IL-6 proteins after LPS treatment of RT4 schwannoma cells under in vitro conditions. Significantly increased levels of NF kappa B, IL-1 beta, pSTAT3, and IL-6 proteins were found 1 h after LPS action indicating their involvement in the initiation of the inflammatory reaction of schwannoma cells. This initiation was induced without increased TLR4 protein expression, but was accompanied by the appearance of TLR4 in early endosomes. The protein levels decreased within the next 6 h of treatment with a subsequent increase of NF kappa B, IL-1 beta, and pSTAT3 after 24 h of LPS treatment. In contrast, continuous decrease of IL-6 over time following LPS treatment was unexpected. Levels of soluble IL-6 protein in the culture medium also decreased with decreasing levels of LPS over 24 h.
引用
收藏
页码:607 / 615
页数:9
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