Fecal microbiota of a dugong (Dugong dugong) in captivity at Toba Aquarium

被引:30
作者
Tsukinowa, Eiko [1 ]
Karita, Shuichi [1 ]
Asano, Shiro [2 ]
Wakai, Yoshihiro [2 ]
Oka, Yukari [2 ]
Furuta, Masami [2 ]
Goto, Masakazu [1 ]
机构
[1] Mie Univ, Grad Sch Bioresources, Tsu, Mie 5148507, Japan
[2] Toba Aquarium, Toba, Mie, Japan
关键词
dugong; eelgrass; fecal microbiota; marine herbivore;
D O I
10.2323/jgam.54.25
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A captive female dugong at Toba Aquarium (Japan) was examined to describe the microbiota of its lower digestive tracts using the molecular-biological technique, a culture-independent method. The phylogenetic analysis of bacterial 16S rRNA genes was conducted for fecal samples, which were taken at 3 different periods. Based on phylogenetic analysis of these sequences, the representatives of six bacterial phyla could be identified: Actinobacteria (0.7%), Bacteroidetes (15%), Firmicutes (83.1%), Lentisphaerae (0.1%), Proteobacteria (0.1%), and Verrucomicrobia (1.0%), suggesting the existence of bacterial species newly found not only in the digestive tract but also in natural field.
引用
收藏
页码:25 / 38
页数:14
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