Growth inhibition and apoptosis induction by alternol in pancreatic carcinoma cells

被引：8
作者：
Cong, Pei-Fang [1 ]
Qu, Ying-Chun [1 ]
Chen, Jie-Peng [2 ]
Duan, Li-Li [2 ]
Lin, Cheng-Jiang [1 ]
Zhu, Xiao-Lin [1 ]
Li-Ling, Jesse [3 ]
Zhang, Mei-Xia [1 ]
机构：
[1] China Med Univ, Lab Metab Dis Res & Drug Dev, Shenyang 110122, Liaoning, Peoples R China
[2] Strand Biotechnol Inst Res, Shantou 515041, Guangdong, Peoples R China
[3] Nanchuan Inst Biol Res, Joint Key Lab Bioresource Res & Utilizat Sichuan, Chongqing 408400, Peoples R China
来源：
WORLD JOURNAL OF GASTROENTEROLOGY | 2015年 / 21卷 / 15期
关键词：
Pancreatic cancer; Alternol; Chemotherapy; Cell cycle; Apoptosis; CANCER-CELLS; COMPOUND ALTERNOL; MUTANT P53; GEMCITABINE; PACLITAXEL; INVASION; THERAPY; PHASE-2; PATHWAY; ARREST;
D O I：
10.3748/wjg.v21.i15.4526
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
AIM: To investigate the effect of alternol on pancreatic cancer cells. METHODS: Pancreatic cancer cells PANC-1 and BxPC3 were treated with various concentrations of alternol for 24, 48 and 72 h. Cell proliferation was measured by cell counting. Cell cycle distribution and mitochondrial membrane potential were determined by flow cytometry. Apoptosis was determined by a TdT-mediated dUTP nick end labeling assay and Hoechst staining. Expression of caspase 3, Bcl-2, p53 and p21 was measured by western blotting. RESULTS: Alternol showed dose-and time-dependent inhibition of the proliferation of PANC-1 and BxPC3 cells in vitro. Alternol induced apoptosis and cell cycle arrest at S phase and decreased mitochondrial membrane potential. Alternol activated caspase 3, upregulated p53 and p21 expression, and downregulated Bcl-2 expression in a dose-dependent manner. CONCLUSION: Our results suggested that alternol is a candidate for treatment of pancreatic cancer.
引用
收藏
页码：4526 / 4535
页数：10
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