Growth inhibition and apoptosis induction by alternol in pancreatic carcinoma cells

被引：8

作者：

Cong, Pei-Fang ^{[1
]}

Qu, Ying-Chun ^{[1
]}

Chen, Jie-Peng ^{[2
]}

Duan, Li-Li ^{[2
]}

Lin, Cheng-Jiang ^{[1
]}

Zhu, Xiao-Lin ^{[1
]}

Li-Ling, Jesse ^{[3
]}

Zhang, Mei-Xia ^{[1
]}

机构：

[1] China Med Univ, Lab Metab Dis Res & Drug Dev, Shenyang 110122, Liaoning, Peoples R China

[2] Strand Biotechnol Inst Res, Shantou 515041, Guangdong, Peoples R China

[3] Nanchuan Inst Biol Res, Joint Key Lab Bioresource Res & Utilizat Sichuan, Chongqing 408400, Peoples R China

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2015年 / 21卷 / 15期

关键词：

Pancreatic cancer; Alternol; Chemotherapy; Cell cycle; Apoptosis; CANCER-CELLS; COMPOUND ALTERNOL; MUTANT P53; GEMCITABINE; PACLITAXEL; INVASION; THERAPY; PHASE-2; PATHWAY; ARREST;

D O I：

10.3748/wjg.v21.i15.4526

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To investigate the effect of alternol on pancreatic cancer cells. METHODS: Pancreatic cancer cells PANC-1 and BxPC3 were treated with various concentrations of alternol for 24, 48 and 72 h. Cell proliferation was measured by cell counting. Cell cycle distribution and mitochondrial membrane potential were determined by flow cytometry. Apoptosis was determined by a TdT-mediated dUTP nick end labeling assay and Hoechst staining. Expression of caspase 3, Bcl-2, p53 and p21 was measured by western blotting. RESULTS: Alternol showed dose-and time-dependent inhibition of the proliferation of PANC-1 and BxPC3 cells in vitro. Alternol induced apoptosis and cell cycle arrest at S phase and decreased mitochondrial membrane potential. Alternol activated caspase 3, upregulated p53 and p21 expression, and downregulated Bcl-2 expression in a dose-dependent manner. CONCLUSION: Our results suggested that alternol is a candidate for treatment of pancreatic cancer.

引用

页码：4526 / 4535

页数：10

共 50 条

[21] Propofol induces apoptosis and increases gemcitabine sensitivity in pancreatic cancer cells in vitro by inhibition of nuclear factor-κB activity [J].

Du, Qi-Hang ;

Xu, Yan-Bing ;

Zhang, Meng-Yuan ;

Yun, Peng ;

He, Chang-Yao .

WORLD JOURNAL OF GASTROENTEROLOGY, 2013, 19 (33) :5485-5492

[22] The effects of Angelica decursiva extract in the inhibition of cell proliferation and in the induction of apoptosis in osteogenic sarcoma cells [J].

Lee, Sook-Young ;

Kim, Chun Sung ;

Cho, Seon-Ho ;

Chun, Hong Sung ;

Kim, Jong-Keun ;

Kim, Do Kyung .

JOURNAL OF MEDICINAL PLANTS RESEARCH, 2009, 3 (04) :241-245

[23] Inhibition of proliferation and induction of apoptosis by γ-tocotrienol in human colon carcinoma HT-29 cells [J].

Xu, Wei-Li ;

Liu, Jia-Ren ;

Liu, Hui-Kun ;

Qi, Gui-Yun ;

Sun, Xiang-Rong ;

Sun, Wen-Guang ;

Chen, Bing-Qing .

NUTRITION, 2009, 25 (05) :555-566

[24] Growth inhibition and apoptosis induction in human hepatoma cells by tanshinone IIA [J].

Tang Zhongzhi ;

Tang Ying ;

Fu Libo .

Current Medical Science, 2003, 23 (2) :166-168

[25] Growth Inhibition and Induction of apoptosis in sarcoma 180 cells by juglone in vivo [J].

Ji Yu Bin ;

Qu Zhong Yuan ;

Zou Xiang ;

Cui Lan ;

Hu Guo Jun .

FBIE: 2008 INTERNATIONAL SEMINAR ON FUTURE BIOMEDICAL INFORMATION ENGINEERING, PROCEEDINGS, 2008, :325-328

[26] Induction of growth inhibition and apoptosis in human uterine leiomyoma cells by isoliquiritigenin [J].

Kim, Dong-chul ;

Ramachandran, Sabarish ;

Baek, Seung-hee ;

Kwon, Sang-Hoon ;

Kwon, Kun-Young ;

Cha, Soon-Do ;

Bae, Insoo ;

Cho, Chi-Heum .

REPRODUCTIVE SCIENCES, 2008, 15 (06) :552-558

[27] Induction of Growth Inhibition and Apoptosis in Human Uterine Leiomyoma Cells by Isoliquiritigenin [J].

Dong-chul Kim ;

Sabarish Ramachandran ;

Seung-hee Baek ;

Sang-Hoon Kwon ;

Kun-Young Kwon ;

Soon-Do Cha ;

Insoo Bae ;

Chi-Heum Cho .

Reproductive Sciences, 2008, 15 :552-558

[28] RNAi-mediated silencing of the Skp-2 gene causes inhibition of growth and induction of apoptosis in human renal carcinoma cells [J].

Lu, Hongsheng ;

Gan, Meifu ;

Cao, Xuequan ;

Chen, Lanxi ;

Yang, Zhaohui ;

Hu, Wei .

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2014, 7 (07) :3845-3852

[29] Growth inhibition and apoptosis induction Sulindac on Human gastric cancer cells [J].

Yun-Lin Wu~1 Bo Sun~1 Xue-Jun Zhang~2 Sheng-Nian Wang~2 Heng-Yi He~2 Min-Min Qiao~1 Jie Zhong~1 Jia-Yu Xu~1 1 Department of Gastroenterology .

World Journal of Gastroenterology, 2001, (06) :796-800

[30] Synergistic Inhibition of Pancreatic Cancer Cell Growth and Migration by Gemcitabine and Withaferin A [J].

Szydlak, Renata .

BIOMOLECULES, 2024, 14 (09)

← 1 2 3 4 5 →