Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells

被引：27

作者：

Carvalho, Pedro P. ^{[1
,2
,3
,5
]}

Wu, Xiying ^{[1
]}

Yu, Gang ^{[1
]}

Dietrich, Marilyn ^{[4
]}

Dias, Isabel R. ^{[2
,3
,5
]}

Gomes, Manuela E. ^{[2
,3
]}

Reis, Rui L. ^{[2
,3
]}

Gimble, Jeffrey M. ^{[1
]}

机构：

[1] Louisiana State Univ Syst, Stem Cell Biol Lab, Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA

[2] Univ Minho, Headquarters European Inst Excellence Tissue Engn, 3Bs Res Grp, Avepark, Guimaraes, Portugal

[3] PT Associated Lab, IBB, Braga, Portugal

[4] Louisiana State Univ, Sch Vet Med, Flow Cytometry Lab, Dept Pathobiol Sci, Baton Rouge, LA 70803 USA

[5] Univ Tras Os Montes & Alto Douro, Dept Vet Sci, Sch Agrarian & Vet Sci, Vila Real, Portugal

来源：

CYTOTHERAPY | 2011年 / 13卷 / 05期

关键词：

STEM-CELLS; IN-VITRO; RECOMBINANT TRYPSIN; BOVINE EMBRYOS; CULTURE; TISSUE;

D O I：

10.3109/14653249.2010.544721

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells using porcine-derived trypsin. We have compared porcine trypsin with animal protein-free products from recombinant bacteria (TrypLE Express; Invitrogene) and corn (TrypZean; Sigma) based on cell yield, viability and immunophenotype. ASC harvested with each trypsin product were comparable.

引用

页码：594 / 597

页数：4

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