LincRNA-p21 exon 1 expression correlates with Cdkn1a expression in vivo

被引：4
|
作者：
Furuhata, Riki [1 ]
Imasaka, Mai [1 ,2 ]
Sugimoto, Michihiko [1 ,3 ]
Yoshinobu, Kumiko [4 ]
Araki, Masatake [4 ]
Araki, Kimi [1 ]
机构：
[1] Kumamoto Univ, Inst Resource Dev & Anal, Div Dev Genet, Kumamoto, Japan
[2] Hyogo Coll Med, Genet, Nishinomiya, Hyogo, Japan
[3] RIKEN, BioResource Res Ctr, Technol & Dev Team Mammalian Genome Dynam, Tsukuba, Ibaraki, Japan
[4] Kumamoto Univ, Inst Resource Dev & Anal, Div Genom, Kumamoto, Japan
来源：
GENES TO CELLS | 2022年 / 27卷 / 01期
基金：
日本学术振兴会;
关键词：
cyclin-dependent kinase inhibitor 1A; enhancer elements; LincRNA; mouse; upregulation; EMBRYONIC STEM-CELLS; GENE-EXPRESSION; CIS; TRANSCRIPTION; ACTIVATION; LNCRNA; RNAS;
D O I：
10.1111/gtc.12906
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
LincRNA-p21 is a long intergenic non-coding RNA (LincRNA) gene reported to activate the transcription of the adjacent Cdkn1a (p21) gene in cis. The importance of the enhancer elements in the LincRNA-p21 gene region has also been reported; however, the involvement of the LincRNA-p21 transcripts in regulating Cdkn1a in vivo is still unclear. In this study, we used a LincRNA-p21-trapped mouse line (LincRNA-p21(Gt)) in which beta geo was inserted into intron 1, and all enhancer elements were retained. In LincRNA-p21(Gt/Gt) mice, the transcription of LincRNA-p21 was repressed due to the beta geo sequence, and the expression of exon 1 of LincRNA-p21 was restored through its deletion or replacement by another sequence, and Cdkn1a expression was also upregulated. Furthermore, regardless of the full-length transcripts, the expression of Cdkn1a correlated with the transcription of the exon 1 of LincRNA-p21. This result indicates that the LincRNA-p21 transcripts are not functional, but the transcriptional activity around exon 1 is important for Cdkn1a expression.
引用
收藏
页码：14 / 24
页数：11
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