Crystallization and preliminary X-ray diffraction analysis of importin-α acomplexed with NLS peptidomimetics

被引：2
|
作者：
Fontes, MRM
Teh, T
Riell, RD
Park, SB
Standaert, RE
Kobe, B
机构：
[1] Univ Estadual Paulista, Dept Fis & Biofis, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
[2] Univ Queensland, Dept Biochem & Mol Biol, Inst Mol Biosci, ARC Special Res Ctr Funct & Appl Genom, Brisbane, Qld 4072, Australia
[3] Univ Queensland, Cooperat Res Ctr Chron Inflammatory Dis, Brisbane, Qld 4072, Australia
[4] Univ Illinois, Dept Chem, Chicago, IL USA
[5] Seoul Natl Univ, Sch Chem, Seoul 151742, South Korea
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2005年 / 1750卷 / 01期
基金：
巴西圣保罗研究基金会;
关键词：
crystallization; X-ray crystallography; importin-alpha; karyopherin-alpha; nuclear localization sequence; NLS peptidomimetic ligand;
D O I：
10.1016/j.bbapap.2005.03.014
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Importin-alpha is the nuclear import receptor that recognizes cargo proteins with nuclear localization sequences (NLSs). Tile study of NLS peptidomimetics can provide a better understanding of the requirements for the molecular recognition of cargo proteins by importin-alpha, and potentially engender a large number of applications in medicine. Importin-a was crystallized with a set of six NLS peptidomimetics, and X-ray diffraction data were collected in the range 2.1-2.5 angstrom resolution. Preliminary electron density calculations show that the ligands are present in the crystals. (c) 2005 Elsevier B.V All rights reserved.
引用
收藏
页码：9 / 13
页数：5
相关论文
共 50 条
  • [41] Crystallization and Preliminary X-Ray Diffraction Studies of a Psychrophilic Iron Superoxide Dismutase from Pseudoalteromonas haloplanktis
    Merlino, Antonello
    Krauss, Irene Russo
    Castellano, Immacolata
    De Vendittis, Emmanuele
    Vergara, Alessandro
    Sica, Filomena
    PROTEIN AND PEPTIDE LETTERS, 2008, 15 (04) : 415 - 418
  • [42] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF HUMAN RANTES
    SHAW, JP
    KRYGER, G
    CLEASBY, A
    WONACOTT, A
    POWER, CA
    PROUDFOOT, AEI
    WELLS, TNC
    JOURNAL OF MOLECULAR BIOLOGY, 1994, 242 (04) : 589 - 590
  • [43] Crystallization and preliminary X-ray diffraction analysis of a myotoxic phospholipase A2 homologue from Bothrops neuwiedi pauloensis venom
    Fontes, MRM
    Soares, AM
    Rodrigues, VM
    Fernandes, AC
    Da Silva, RJ
    Giglio, JR
    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1999, 1432 (02): : 393 - 395
  • [44] Crystallization and preliminary X-ray analysis of the dsDNA bacteriophage HK97 mature empty capsid
    Wikoff, WR
    Duda, RL
    Hendrix, RW
    Johnson, JE
    VIROLOGY, 1998, 243 (01) : 113 - 118
  • [45] Purification, crystallization, X-ray diffraction analysis and phasing of a fungal 17β-hydroxysteroid dehydrogenase
    Höög, JO
    Staab, CA
    Hedberg, JJ
    Grafström, RC
    Büdefeld, T
    Cassetta, A
    Rizner, TL
    Kristan, K
    Stojan, J
    Lamba, D
    Enzymology and Molecular Biology of Carbonyl Metabolism 12, 2006, 12 : 360 - 365
  • [46] Cloning, purification, crystallization, and preliminary X-ray diffraction analysis of cystathionine gamma-synthase from E-coli
    Wahl, MC
    Huber, R
    Prade, L
    Marinkovic, S
    Messerschmidt, A
    Clausen, T
    FEBS LETTERS, 1997, 414 (03) : 492 - 496
  • [47] Crystallization and preliminary X-ray diffraction studies of a 40 kDa calcium binding protein specifically expressed in plasmodia of Physarum polycephalum
    Iwasaki, W
    Sasaki, H
    Nakamura, A
    Kohama, K
    Tanokura, M
    JOURNAL OF BIOCHEMISTRY, 1999, 126 (01) : 7 - 9
  • [48] Crystallization and preliminary X-ray diffraction studies of two myotoxic Lys49-phospholipases A2 complexed with α-tocopherol
    dos Santos, JI
    Takeda, AAS
    Marchi-Salvador, DP
    Soares, AM
    Fontes, MRM
    PROTEIN AND PEPTIDE LETTERS, 2005, 12 (08) : 819 - 822
  • [49] Crystallization and preliminary x-ray crystallographic analysis of squalene-hopene cyclase from Alicyclobacillus acidocaldarius
    Wendt, KU
    Feil, C
    Lenhart, A
    Poralla, K
    Schulz, GE
    PROTEIN SCIENCE, 1997, 6 (03) : 722 - 724
  • [50] Crystallization and preliminary X-ray studies of porcine kidney aminoacylase Ⅰ
    ZHAOHaiyan
    2. Department of Biological Science and Biotechnology
    Chinese Science Bulletin, 1997, (20) : 1750 - 1752
12345