Crystallization and preliminary X-ray diffraction analysis of importin-α acomplexed with NLS peptidomimetics

被引：2
|
作者：
Fontes, MRM
Teh, T
Riell, RD
Park, SB
Standaert, RE
Kobe, B
机构：
[1] Univ Estadual Paulista, Dept Fis & Biofis, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
[2] Univ Queensland, Dept Biochem & Mol Biol, Inst Mol Biosci, ARC Special Res Ctr Funct & Appl Genom, Brisbane, Qld 4072, Australia
[3] Univ Queensland, Cooperat Res Ctr Chron Inflammatory Dis, Brisbane, Qld 4072, Australia
[4] Univ Illinois, Dept Chem, Chicago, IL USA
[5] Seoul Natl Univ, Sch Chem, Seoul 151742, South Korea
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2005年 / 1750卷 / 01期
基金：
巴西圣保罗研究基金会;
关键词：
crystallization; X-ray crystallography; importin-alpha; karyopherin-alpha; nuclear localization sequence; NLS peptidomimetic ligand;
D O I：
10.1016/j.bbapap.2005.03.014
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Importin-alpha is the nuclear import receptor that recognizes cargo proteins with nuclear localization sequences (NLSs). Tile study of NLS peptidomimetics can provide a better understanding of the requirements for the molecular recognition of cargo proteins by importin-alpha, and potentially engender a large number of applications in medicine. Importin-a was crystallized with a set of six NLS peptidomimetics, and X-ray diffraction data were collected in the range 2.1-2.5 angstrom resolution. Preliminary electron density calculations show that the ligands are present in the crystals. (c) 2005 Elsevier B.V All rights reserved.
引用
收藏
页码：9 / 13
页数：5
相关论文
共 50 条
  • [31] Crystallization and preliminary X-ray analysis of human transglutaminase 3 from zymogen to active form
    Kim, HC
    Nemes, Z
    Idler, WW
    Hyde, CC
    Steinert, PM
    Ahvazi, B
    JOURNAL OF STRUCTURAL BIOLOGY, 2001, 135 (01) : 73 - 77
  • [32] Vinorine synthase from Rauvolfia:: the first example of crystallization and preliminary X-ray diffraction analysis of an enzyme of the BAHD superfamily
    Ma, XY
    Koepke, J
    Bayer, A
    Linhard, V
    Fritzsch, G
    Zhang, B
    Michel, H
    Stöckigt, J
    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2004, 1701 (1-2): : 129 - 132
  • [33] Crystallization and preliminary X-ray diffraction analysis of a recombinant bacterial heme oxygenase (Hmu O) from Corynebacterium diphtheriae
    Chu, GC
    Park, SY
    Shiro, Y
    Yoshida, T
    Ikeda-Saito, M
    JOURNAL OF STRUCTURAL BIOLOGY, 1999, 126 (02) : 171 - 174
  • [34] Crystallization and preliminary X-ray analysis of a DNA primase from hyperthermophilic archaeon Pyrococcus horikoshii
    Ito, N
    Nureki, O
    Shirouzu, M
    Yokoyama, S
    Hanaoka, F
    JOURNAL OF BIOCHEMISTRY, 2001, 130 (06) : 727 - 730
  • [35] Crystallization and preliminary x-ray crystallographic analysis of spruce budworm antifreeze protein
    Graether, SP
    Ye, QL
    Davies, PL
    Jia, ZC
    JOURNAL OF STRUCTURAL BIOLOGY, 1999, 126 (01) : 72 - 75
  • [36] Crystallization and preliminary X-ray crystallographic analysis of human geranylgeranyl pyrophosphate synthase
    CHEN Yushu
    Progress in Natural Science, 2006, (12) : 1353 - 1356
  • [37] Crystallization and preliminary X-ray analysis of aldehyde dehydrogenase from Vibrio harveyi
    Croteau, N
    Vedadi, M
    Delarge, M
    Meighen, E
    AbuAbed, M
    Howell, PL
    Vrielink, A
    PROTEIN SCIENCE, 1996, 5 (10) : 2130 - 2132
  • [38] Crystallization and preliminary X-ray analysis of restriction endonuclease FokI bound to DNA
    Hirsch, JA
    Wah, DA
    Dorner, LF
    Schildkraut, I
    Aggarwal, AK
    FEBS LETTERS, 1997, 403 (02) : 136 - 138
  • [39] Crystallization and preliminary X-ray crystallographic analysis of human geranylgeranyl pyrophosphate synthase
    Chen Yushu
    Li Xiaofeng
    Lou Zhiyong
    Wang Weina
    Pang Hai
    Rao Zihe
    PROGRESS IN NATURAL SCIENCE, 2006, 16 (12) : 1353 - 1356
  • [40] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF DIALKYLGLYCINE DECARBOXYLASE, A DECARBOXYLATING TRANSAMINASE
    TONEY, MD
    KELLER, JW
    PAUPTIT, RA
    JAEGER, J
    WISE, MK
    SAUDER, U
    JANSONIUS, JN
    JOURNAL OF MOLECULAR BIOLOGY, 1991, 222 (04) : 873 - 875
12345