Biomolecule-stabilized Au nanoclusters as a fluorescence probe for sensitive detection of glucose

被引：247
作者：
Jin, Lihua [1 ]
Shang, Li [1 ]
Guo, Shaojun [1 ]
Fang, Youxing [1 ]
Wen, Dan [1 ]
Wang, Li [1 ]
Yin, Jianyuan [1 ]
Dong, Shaojun [1 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, Grad Sch, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2011年 / 26卷 / 05期
基金：
中国国家自然科学基金;
关键词：
Biomolecule; Au nanoclusters; Hydrogen peroxide; Glucose; Fluorescence probe; CDTE QUANTUM DOTS; GOLD NANOPARTICLES; CLUSTERS; CORE;
D O I：
10.1016/j.bios.2010.08.019
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
In this work, biomolecule-stabilized Au nanoclusters were demonstrated as a novel fluorescence probe for sensitive and selective detection of glucose. The fluorescence of Au nanoclusters was found to be quenched effectively by the enzymatically generated hydrogen peroxide (H2O2). By virtue of the specific response, the present assay allowed for the selective determination of glucose in the range of 1.0 x 10(-5) M to 0.5 x 10(-3) M with a detection limit of 5.0 x 10(-6) M. The absorption spectroscopy, X-ray photoelectron spectroscopy (XPS) and fluorescence decay studies were then performed to discuss the quenching mechanism. In addition, we demonstrated the application of the present approach in real serum samples, which suggested its great potential for diagnostic purposes. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码：1965 / 1969
页数：5
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