Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum

被引：32
作者：
Durand, R
Eslahpazire, J
Jafari, S
Delabre, JF
Marmorat-Khuong, A
di Piazza, JP
Le Bras, J
机构：
[1] Hop Bichat Claude Bernard, Parasitol Lab, Assistance Publ Hop Paris, F-75877 Paris 18, France
[2] Univ Paris 05, Ctr Natl Reference Chimiosensibil Paludisme, F-75877 Paris, France
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2000年 / 44卷 / 12期
关键词：
D O I：
10.1128/AAC.44.12.3461-3464.2000
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point mutation in Plasmodium falciparum. One hundred African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a promising tool for epidemiological studies.
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页码：3461 / 3464
页数：4
相关论文
共 25 条
[1]   Plasmodium falciparum: Molecular characterization of multidrug-resistant Cambodian isolates [J].
Basco, LK ;
dePecoulas, PE ;
LeBras, J ;
Wilson, CM .
EXPERIMENTAL PARASITOLOGY, 1996, 82 (02) :97-103
[2]   POINT MUTATIONS IN THE DIHYDROFOLATE-REDUCTASE THYMIDYLATE SYNTHASE GENE AND PYRIMETHAMINE AND CYCLOGUANIL RESISTANCE IN PLASMODIUM-FALCIPARUM [J].
BASCO, LK ;
DEPECOULAS, PE ;
WILSON, CM ;
LEBRAS, J ;
MAZABRAUD, A .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1995, 69 (01) :135-138
[3]   BEYOND CHLOROQUINE - IMPLICATIONS OF DRUG-RESISTANCE FOR EVALUATING MALARIA THERAPY EFFICACY AND TREATMENT POLICY IN AFRICA [J].
BLOLAND, PB ;
LACKRITZ, EM ;
KAZEMBE, PN ;
WERE, JBO ;
STEKETEE, R ;
CAMPBELL, CC .
JOURNAL OF INFECTIOUS DISEASES, 1993, 167 (04) :932-937
[4]   PLASMODIUM-FALCIPARUM - DETECTION OF ANTIFOLATE RESISTANCE BY MUTATION-SPECIFIC RESTRICTION ENZYME DIGESTION [J].
DEPECOULAS, PE ;
BASCO, LK ;
ABDALLAH, B ;
DJE, MK ;
LEBRAS, J ;
MAZABRAUD, A .
EXPERIMENTAL PARASITOLOGY, 1995, 80 (03) :483-487
[5]   Analysis of κ and ω repeats of the cg2 gene and chloroquine susceptibility in isolates of Plasmodium fulciparum from sub-Saharan Africa [J].
Durand, R ;
Gabbett, E ;
Di Piazza, JP ;
Delabre, JF ;
Le Bras, J .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1999, 101 (1-2) :185-197
[6]  
Durand R, 1999, ANN TROP MED PARASIT, V93, P25, DOI 10.1080/00034989958762
[7]  
DURAND R, 1997, T R SOC TROP MED HYG, V85, P33
[8]   Monitoring the amplification of CATCH, a 3SR based cooperatively coupled isothermal amplification system, by fluorimetric methods [J].
Ehricht, R ;
Kirner, T ;
Ellinger, T ;
Foerster, P ;
McCaskill, JS .
NUCLEIC ACIDS RESEARCH, 1997, 25 (22) :4697-4699
[9]  
FERONE R, 1977, B WORLD HEALTH ORGAN, V55, P291
[10]  
Giesendorf BAJ, 1998, CLIN CHEM, V44, P482
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