Duration of sample storage dramatically alters expression of the human immunodeficiency virus coreceptors CXCR4 and CCR5

被引：15

作者：

Shalekoff, S

Tiemessen, CT

机构：

[1] Univ Witwatersrand, Dept Virol, ZA-2050 Witwatersrand, South Africa

[2] Natl Inst Virol, AIDS Virus Res Unit, Johannesburg, South Africa

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2001年 / 8卷 / 02期

关键词：

D O I：

10.1128/CDLI.8.2.432-436.2001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Expression of the chemokine receptors CXCR4 and CCR5 was monitored using EDTA-anticoagulated whole blood held for different time periods prior to fluorescent-antibody staining. When left overnight CXCR4 expression on leukocytes was substantially increased, whereas CCR5 expression was reduced. The results were similar when heparin and acid-citrate-dextrose were used as anticoagulants.

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页码：432 / 436

页数：5

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