Preparative isolation and purification of acteoside and 2′-acetyl acteoside from Cistanches salsa (C.A.!Mey.) G.!Beck by highspeed counter-current chromatography

被引：81
作者：
Li, L
Yang, FQ
Zhang, TY
Tu, PF
Wu, LJ
Ito, YC
机构：
[1] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA
[2] Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
[3] Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China
[4] Shenyang Pharmaceut Univ, Shenyang 110015, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY A | 2001年 / 912卷 / 01期
关键词：
counter-current chromatography; Cistanches salsa; pharmaceutical analysis; plant materials; preparative chromatography; acteoside; acetylacteoside; phenylethanoids; glycosides;
D O I：
10.1016/S0021-9673(01)00583-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-speed counter-current chromatography (HSCCC) was applied to the separation and purification of phenylethanoid glycosides (PhGs) acteoside and 2 ' -acetylacteoside from Cistanches salsa (C.A. Mey) G. Beck with a quaternary two-phase solvent system composed of ethyl acetate-n-butanol-ethanol-water (4:0.6:0.6:5, v/v). HPLC analyses of the CCC fractions revealed that the two main PhGs were over 98% purity. Their chemical structures were identified by LH NMR, C-13 NMR and MS. (C) 2001 Elsevier Science B.V. All rights reserved.
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页码：181 / 185
页数：5
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