Preparative isolation and purification of acteoside and 2′-acetyl acteoside from Cistanches salsa (C.A.!Mey.) G.!Beck by highspeed counter-current chromatography

被引：83

作者：

Li, L

Yang, FQ

Zhang, TY

Tu, PF

Wu, LJ

Ito, YC

机构：

[1] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

[2] Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

[3] Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China

[4] Shenyang Pharmaceut Univ, Shenyang 110015, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 912卷 / 01期

关键词：

counter-current chromatography; Cistanches salsa; pharmaceutical analysis; plant materials; preparative chromatography; acteoside; acetylacteoside; phenylethanoids; glycosides;

D O I：

10.1016/S0021-9673(01)00583-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed counter-current chromatography (HSCCC) was applied to the separation and purification of phenylethanoid glycosides (PhGs) acteoside and 2 ' -acetylacteoside from Cistanches salsa (C.A. Mey) G. Beck with a quaternary two-phase solvent system composed of ethyl acetate-n-butanol-ethanol-water (4:0.6:0.6:5, v/v). HPLC analyses of the CCC fractions revealed that the two main PhGs were over 98% purity. Their chemical structures were identified by LH NMR, C-13 NMR and MS. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：181 / 185

页数：5

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[2]

ITO Y, 1986, CRIT REV ANAL CHEM, V17, P65, DOI 10.1080/10408348608542792

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