Direct protein microarray fabrication using a hydrogel "stamper"

被引：141

作者：

Martin, BD ^{[1
]}

Gaber, BP ^{[1
]}

Patterson, CH ^{[1
]}

Turner, DC ^{[1
]}

机构：

[1] USN, Res Lab, Washington, DC 20375 USA

来源：

LANGMUIR | 1998年 / 14卷 / 15期

关键词：

D O I：

10.1021/la9713311

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Micropatterned arrays of active proteins are vital to the next generation of high-throughput multiplexed biosensors and advanced medical diagnostics. We have developed a simple method for fabricating antibody arrays using a micromolded hydrogel "stamper" and an aminosilylated receiving surface. The stamping procedure permits direct protein deposition and micropatterning while avoiding cross-contamination of separate patterned regions. Three different antibodies were stamped in adjacent arrays of 50-80 mu m circular areas with retention of activity. I-125 labeling and atomic force microscopy studies showed that the stamper deposited protein as a submonolayer. The fluorescent signal-to-background ratio of labeled bound antigen was greater than 25:1.

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收藏

页码：3971 / 3975

页数：5

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