Development and application of an indirect ELISA for the serological detection of duck Tembusu virus infection based on the NS1 protein antigen

被引：12
|
作者：
Zhou, Qi [1 ]
Bi, Zhuangli [1 ,2 ]
Yin, Dongdong [1 ,3 ]
Gu, Xiangxue [1 ]
Xu, Zejun [1 ]
Huang, Rong [1 ]
Xing, Xue [1 ]
Qi, Kezong [3 ]
Wang, Guijun [1 ,3 ]
机构：
[1] Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230036, Anhui, Peoples R China
[2] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China
[3] Anhui Prov Key Lab Vet Pathobiol & Dis Control, Hefei 230036, Anhui, Peoples R China
来源：
ARCHIVES OF VIROLOGY | 2020年 / 165卷 / 03期
关键词：
LINKED-IMMUNOSORBENT-ASSAY; JAPANESE ENCEPHALITIS-VIRUS; WEST-NILE-VIRUS; PCR ASSAY; ANTIBODIES; FLAVIVIRUS; DISEASE; PHASE; STEP;
D O I：
10.1007/s00705-019-04495-4
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Duck Tembusu virus (DTMUV) has caused significant economic losses in China since 2010. However, there is still a lack of effective methods to diagnose the disease caused by this virus, and especially to differentiate infection from vaccination. In this study, we established a novel indirect enzyme-linked immunosorbent assay (iELISA) and performed a retrospective serological survey for DTMUV in Anhui province, China. Our results show that the iELISA displayed high specificity sensitivity, and with no serological cross-reaction with other duck pathogens. These findings indicate that the newly developed iELISA could be a useful screening tool for large-scale monitoring of the epidemiology of DTMUV infection in ducks.
引用
收藏
页码：709 / 714
页数：6
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