The mutagenic versus protective role of vitamin A on the induction of chromosomal aberration in human lymphocyte cultures

The present study was carried out to evaluate the role of vitamin A (VA) on the induction of chromosomal aberrations (CA) in lymphocyte culture system and to investigate its modulating effect on chromosomal damage induced by gamma irradiation. Lymphocyte cultures from five healthy normal adult males were either treated with VA at a dose level of 2.0, 8.0 or 24.0 mu g/ml or exposed to gamma-irradiation of 3.0 Gy, then followed immediately by a treatment with one of the above mentioned doses of VA. Non-treated cultures and cultures exposed to gamma-irradiation served as control for the two sets of experiments. Cultures were set up in duplicates and incubated for 48 h for assessment of CA. Treatment with VA alone increased CA demonstrating a dose-response effect. Addition of VA to gamma-irradiated cultures resulted in an inverse protective effect as the low dose of 2 mu g/ml reduced the CA induced by radiation to about 1/3 rd whereas a dose of 8 mu g/ml had a protective effect of 40% of the total damage and the large dose of 24 mu g/ml had no or little effect. These results suggest that a proportion of the added VA may interfere with the radiation induced free radicals and other reactive metabolites which elevate CA. On the other hand, excessive amounts of VA increased toxicity and reduced effect on repair enzymes. (C) 1998 Elsevier Science B.V. All rights reserved.