Nonscanning large-area Raman imaging for ex vivo/in vivo skin cancer discrimination

被引：14
作者：
Schmaelzlin, Elmar [1 ]
Moralejo, Benito [1 ]
Gersonde, Ingo [1 ,2 ]
Schleusener, Johannes [3 ,4 ]
Darvin, Maxim E. [3 ,4 ]
Thiede, Gisela [3 ,4 ]
Roth, Martin M. [1 ,5 ]
机构：
[1] InnoFSPEC Potsdam, Leibniz Inst Astrophys Potsdam, Potsdam, Germany
[2] Univ Potsdam, Phys Chem, InnoFSPEC Potsdam, Potsdam, Germany
[3] Humboldt Univ, Charite Univ Med Berlin, Freie Univ Berlin, Berlin, Germany
[4] Berlin Inst Hlth, Dept Dermatol Venerol & Allergol, Ctr Expt & Appl Cutaneous Physiol, Berlin, Germany
[5] Univ Potsdam, Inst Phys & Astron, Potsdam, Germany
来源：
JOURNAL OF BIOMEDICAL OPTICS | 2018年 / 23卷 / 10期
关键词：
Raman spectroscopy; cancer diagnosis; Raman imaging; multichannel; astronomy; epidermis; dermis; IN-VIVO; POSITIVE MARGINS; SPECTROSCOPY; FLUORESCENCE; CAROTENOIDS; SENSITIVITY; DIAGNOSIS; RESECTION; SURGERY; CELLS;
D O I：
10.1117/1.JBO.23.10.105001
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Imaging Raman spectroscopy can be used to identify cancerous tissue. Traditionally, a step-by-step scanning of the sample is applied to generate a Raman image, which, however, is too slow for routine examination of patients. By transferring the technique of integral field spectroscopy (IFS) from astronomy to Raman imaging, it becomes possible to record entire Raman images quickly within a single exposure, without the need for a tedious scanning procedure. An IFS-based Raman imaging setup is presented, which is capable of measuring skin ex vivo or in vivo. It is demonstrated how Raman images of healthy and cancerous skin biopsies were recorded and analyzed. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.
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页数：11
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共 43 条
  • [1] STATISTICS NOTES - DIAGNOSTIC-TESTS-1 - SENSITIVITY AND SPECIFICITY .3.
    ALTMAN, DG
    BLAND, JM
    [J]. BRITISH MEDICAL JOURNAL, 1994, 308 (6943) : 1552 - 1552
  • [2] The second generation VLT instrument MUSE:: Science drivers and instrument design
    Bacon, R
    Bauer, S
    Bower, R
    Cabrit, S
    Cappellari, M
    Carollo, M
    Combes, FO
    Davies, R
    Delabre, B
    Dekker, H
    Devriendt, J
    Djidel, S
    Duchateau, M
    Dubois, JP
    Emsellem, E
    Ferruit, P
    Franx, M
    Gilmore, G
    Guiderdoni, B
    Hénault, F
    Hubin, N
    Jungwiert, B
    Kelz, A
    Le Louarn, M
    Lewis, I
    Lizon, JL
    Mc Dermid, R
    Morris, S
    Laux, U
    Le Fevre, O
    Lantz, B
    Lilly, S
    Lynn, J
    Pasquin, L
    Pécontal, A
    Popovic, PPD
    Quirrenbach, A
    Reiss, R
    Roth, M
    Steinmetz, M
    Stuik, R
    Wisotzki, L
    de Zeeuw, T
    [J]. GROUND-BASED INSTRUMENTATION FOR ASTRONOMY, PTS 1-3, 2004, 5492 : 1145 - 1149
  • [3] STANDARD NORMAL VARIATE TRANSFORMATION AND DE-TRENDING OF NEAR-INFRARED DIFFUSE REFLECTANCE SPECTRA
    BARNES, RJ
    DHANOA, MS
    LISTER, SJ
    [J]. APPLIED SPECTROSCOPY, 1989, 43 (05) : 772 - 777
  • [4] Combined Raman and autofluorescence ex vivo diagnostics of skin cancer in near- infrared and visible regions
    Bratchenko, Ivan A.
    Artemyev, Dmitry N.
    Myakinin, Oleg O.
    Khristoforova, Yulia A.
    Moryatov, Alexander A.
    Kozlov, Sergey V.
    Zakharov, Valery P.
    [J]. JOURNAL OF BIOMEDICAL OPTICS, 2017, 22 (02)
  • [5] Fiber array based hyperspectral Raman imaging for chemical selective analysis of malaria-infected red blood cells
    Brueckner, Michael
    Becker, Katja
    Popp, Juergen
    Frosch, Torsten
    [J]. ANALYTICA CHIMICA ACTA, 2015, 894 : 76 - 84
  • [6] A robust method for automated background subtraction of tissue fluorescence
    Cao, Alex
    Pandya, Abhilash K.
    Serhatkulu, Gulay K.
    Weber, Rachel E.
    Dai, Houbei
    Thakur, Jagdish S.
    Naik, Vaman M.
    Naik, Ratna
    Auner, Gregory W.
    Rabah, Raja
    Freeman, D. Carl
    [J]. JOURNAL OF RAMAN SPECTROSCOPY, 2007, 38 (09) : 1199 - 1205
  • [7] Depth profiles of hydrogen bound water molecule types and their relation to lipid and protein interaction in the human stratum corneum in vivo
    Choe, ChunSik
    Lademann, Juergen
    Darvin, Maxim E.
    [J]. ANALYST, 2016, 141 (22) : 6329 - 6337
  • [8] Predicting positive margins in resection of cutaneous melanoma of the head and neck
    Christophel, J. Jared
    Johnson, Andrew K.
    McMurry, Timothy L.
    Park, Stephen S.
    Levine, Paul A.
    [J]. LARYNGOSCOPE, 2013, 123 (03) : 683 - 688
  • [9] In vivo distribution of carotenoids in different anatomical locations of human skin: comparative assessment with two different Raman spectroscopy methods
    Darvin, Maxim E.
    Fluhr, Joachim W.
    Caspers, Peter
    van der Pool, Andre
    Richter, Heike
    Patzelt, Alexa
    Sterry, Wolfram
    Lademann, Juergen
    [J]. EXPERIMENTAL DERMATOLOGY, 2009, 18 (12) : 1060 - 1063
  • [10] Real-time Imaging of the Resection Bed Using a Handheld Probe to Reduce Incidence of Microscopic Positive Margins in Cancer Surgery
    Erickson-Bhatt, Sarah J.
    Nolan, Ryan M.
    Shemonski, Nathan D.
    Adie, Steven G.
    Putney, Jeffrey
    Darga, Donald
    McCormick, Daniel T.
    Cittadine, Andrew J.
    Zysk, Adam M.
    Marjanovic, Marina
    Chaney, Eric J.
    Monroy, Guillermo L.
    South, Fredrick A.
    Cradock, Kimberly A.
    Liu, Z. George
    Sundaram, Magesh
    Ray, Partha S.
    Boppart, Stephen A.
    [J]. CANCER RESEARCH, 2015, 75 (18) : 3706 - 3712
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