Effectiveness of Cissampelos sympodialis and its isolated alkaloid warifteine in airway hyperreactivity and lung remodeling in a mouse model of asthma

被引：32

作者：

Bezerra-Santos, Claudio R. ^{[2
]}

Vieira-de-Abreu, Adriana ^{[1
]}

Vieira, Giciane Carvalho ^{[3
]}

Filho, Jaime R. ^{[1
,2
]}

Barbosa-Filho, Jose Maria ^{[4
]}

Pires, Ana Lucia ^{[5
]}

Martins, Marco Aurelio ^{[5
]}

Souza, Heitor S. ^{[6
]}

Bandeira-Melo, Christianne ^{[7
]}

Bozza, Patricia T. ^{[1
]}

Piuvezam, Marcia R. ^{[2
]}

机构：

[1] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Imunofarmacol, BR-21045900 Rio De Janeiro, Brazil

[2] Univ Fed Paraiba, Dept Fisiol & Patol, Lab Imunofarmacol, BR-58051970 Joao Pessoa, Paraiba, Brazil

[3] Univ Fed Paraiba, Dept Morfol, Lab Imunofarmacol, BR-58051970 Joao Pessoa, Paraiba, Brazil

[4] Univ Fed Paraiba, Dept Ciencias Farmaceut, Lab Fitoquim, BR-58051970 Joao Pessoa, Paraiba, Brazil

[5] Fundacao Oswaldo Cruz, Dept Fisiol & Farmacodinam, Lab Inflamacao, BR-21045900 Rio De Janeiro, Brazil

[6] Univ Fed Rio de Janeiro, Hosp Univ Clementino Fraga Filho, Lab Multidisciplinar, Rio De Janeiro, Brazil

[7] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Inflamacao, BR-21941 Rio De Janeiro, Brazil

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2012年 / 13卷 / 02期

关键词：

Cissampelos sympodialis; Warifteine; Airway hyperreactivity; Airway remodeling; Interleukin-13; EICHL; MENISPERMACEAE; INFLAMMATION; EOSINOPHILS; INCREASES; EXTRACT; LEAVES;

D O I：

10.1016/j.intimp.2012.03.014

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background: Cissampelos sympodialis Eichl. (Menispermaceae) is a plant found in Northeastern and Southeast of Brazil and hot water infusion of C sympodialis root bark is largely used in the indigenous and folk medicine to treat several inflammatory disorders, including asthma. Asthma is a chronic inflammatory allergic disease characterized by airway hyperreactivity (AHR), eosinophil tissue infiltration and lung remodeling. The aim of this study was to evaluate the therapeutic effect of C sympodialis and its isolated alkaloid warifteine on allergen triggered airway hyperreactivity (AHR) and lung remodeling in murine model of asthma. Methodology/principal findings: The oral pre-treatment with C. sympodialis or warifteine inhibited allergen-induced AHR to inhaled methacholine and IL-13 levels in the bronchoalveolar lavage (BAL). In order to investigate the therapeutic potential of C. sympodialis and warifteine, animals were treated 1 h after the last ovalbumin (OVA) challenge in sensitized animals. Similarly to the pre-treatment, post-treatment with warifteine was effective to inhibit significantly AHR to inhaled methacholine and to reduce IL-13 levels in the BAL In addition, oral pre- or post-treatments with C sympodialis or warifteine reduced OVA-induced eosinophil tissue infiltration, mucus production and subepithelial fibrosis to values similar to nonallergic controls. Conclusions: Our data show the anti-allergic and immunoregulatory properties of C. sympodialis, acting mostly through the active compound warifteine, to inhibit the airway hyperreactivity and lung remodeling through a mechanism at least partially dependent of IL-13 and eosinophil inhibition. Therefore placing warifteine as an interesting therapeutic candidate in allergic inflammation and corroborating the folk medicine use of C. sympodialis as anti-allergic plant. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：148 / 155

页数：8

共 26 条

[1] Eosinophils: Offenders or General Bystanders in Allergic Airway Disease and Pulmonary Immunity? [J].