Iron overload as a risk factor for poor graft function following allogeneic hematopoietic stem cell transplantation

被引:4
|
作者
Wu, Xue-Qiong [1 ]
Lin, Kang-Ni [1 ]
Chen, Min-Min [1 ]
Jiang, Pei-Fang [1 ]
Zhang, Yu-Xin [1 ]
Chen, Yong-Quan [1 ]
Chen, Qiu-Ru [1 ]
Xiao, Min [1 ]
Zhu, Hao-Jie [1 ]
Issa, Hajji Ally [1 ]
Chen, Shao-Zhen [1 ]
Luo, Xiao-Feng [1 ]
Ren, Jin-Hua [1 ]
Li, Qian [2 ]
Zeng, Yan-Ling [1 ]
Xu, Jing-Jing [1 ]
Lin, Yi-Feng [1 ]
Zheng, Rong [1 ]
Zheng, Zhi-Hong [1 ]
Chen, Zhi-Zhe [1 ]
Hu, Jian-Da [1 ]
Yang, Ting [1 ]
机构
[1] Fujian Med Univ, Fujian Prov Key Lab Hematol, Fujian Inst Hematol, Dept Hematol,Union Hosp, 29 Xinquan Rd, Fuzhou 350001, Fujian, Peoples R China
[2] Fujian Med Univ, Affiliated Nanping Hosp 1, Dept Hematol, Nanping, Fujian, Peoples R China
基金
中国国家自然科学基金;
关键词
allogeneic hematopoietic stem cell transplantation; bone marrow microenvironment; hematopoietic reconstruction; iron overload; poor graft function; BONE-MARROW; ASSOCIATION; SURVIVAL; NICHE;
D O I
10.1002/kjm2.12238
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Hematological malignancies are increasingly treated with allogeneic hematopoietic stem cell transplantation (allo-HSCT). Unfortunately, iron overload is a frequent adverse effect of allo-HSCT and is associated with poor prognosis. In the present study, we investigated hematopoiesis in iron-overloaded mice and elucidated the effects of iron overload on the bone marrow (BM) microenvironment. Iron-overloaded BALB/C mice were generated by injecting 20 mg/mL saccharated iron oxide intraperitoneally. Hematoxylin-eosin staining was performed to evaluate the effects of an iron overload in mice. BM cells obtained from C57BL/6 mice were transplanted into irradiated BALB/C mice (whole-body irradiation of 4 Gy, twice with a 4-hours interval) by tail vein injection. Two weeks after allo-HSCT, the hematopoietic reconstitution capacity was evaluated in recipients by colony-forming assays. Histopathological examinations showed brown-stained granular deposits, irregularly arranged lymphocytes in the liver tissues, and blue-stained blocks in the BM collected from mice received injections of high-dose saccharated iron oxide (20 mg/mL). Iron-overloaded mice showed more platelets, higher-hemoglobin (HGB) concentration, fewer granulocyte-macrophage colony-forming units (CFU-GM), erythrocyte colony-forming units (CFU-E), and mixed granulocyte/erythrocyte/monocyte/megakaryocyte colony-forming units (CFU-mix) than healthy mice. Iron-overloaded recipients presented with reduced erythrocytes and HGB concentration in peripheral blood, along with decreased marrow stroma cells, CFU-GM, CFU-E, and CFU-mix relative to healthy recipients. Taken together, our findings demonstrate that iron overload might alter the number of red blood cells after transplantation in mice by destroying the BM microenvironment, thereby affecting the recovery of BM hematopoietic function.
引用
收藏
页码:825 / 833
页数:9
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