mRNA-Selective Translation Induced by FSH in Primary Sertoli Cells

被引:26
作者
Musnier, Astrid [1 ,2 ,3 ,4 ]
Leon, Kelly [1 ,2 ,3 ,4 ]
Morales, Julia [5 ,6 ,7 ]
Reiter, Eric [1 ,2 ,3 ,4 ]
Boulo, Thomas [1 ,2 ,3 ,4 ]
Costache, Vlad [5 ,6 ,7 ]
Vourc'h, Patrick [8 ,9 ]
Heitzler, Domitille [1 ,2 ,3 ,4 ]
Oulhen, Nathalie [5 ,6 ,7 ]
Poupon, Anne [1 ,2 ,3 ,4 ]
Boulben, Sandrine [5 ,6 ,7 ]
Cormier, Patrick
Crepieux, Pascale
机构
[1] INRA, BIOS Grp, Unite Mixte Rech 85, Unite Physiol Reprod & Comportements, F-37380 Nouzilly, France
[2] CNRS, Unite Mixte Rech 7247, F-37380 Nouzilly, France
[3] Inst Francais du Cheval & Equitat, F-37380 Nouzilly, France
[4] Univ Tours, F-37041 Tours, France
[5] Univ Paris 06, Unite Mixte Rech Mer & Sante 7150, Equipe Traduct Cycle Cellulaire & Dev, Stn Biol Roscoff, F-29239 Roscoff, France
[6] CNRS, Unite Mixte Rech Mer & Sante 7150, Stn Biol Roscoff, F-29239 Roscoff, France
[7] Univ Europeenne Bretagne, F-29239 Roscoff, France
[8] Univ Tours, CNRS, Equipe Rech Labellisee 3106, Unite Mixte Rech,INSERM,U930, F-37000 Tours, France
[9] Ctr Hosp Reg Univ Tours, Lab Biochim & Biol Mol, F-37000 Tours, France
关键词
FOLLICLE-STIMULATING-HORMONE; CAP-DEPENDENT TRANSLATION; PROTEIN-SYNTHESIS; RAT SERTOLI; GENE-EXPRESSION; HIERARCHICAL PHOSPHORYLATION; MAMMALIAN TARGET; IN-VITRO; INITIATION; RECEPTOR;
D O I
10.1210/me.2011-1267
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
FSH is a key hormonal regulator of Sertoli cell secretory activity, required to optimize sperm production. To fulfil its biological function, FSH binds a G protein-coupled receptor, the FSH-R. The FSH-R-transduced signaling network ultimately leads to the transcription or down-regulation of numerous genes. In addition, recent evidence has suggested that FSH might also regulate protein translation. However, this point has never been demonstrated conclusively yet. Here we have addressed this issue in primary rat Sertoli cells endogenously expressing physiological levels of FSH-R. We observed that, within 90 min of stimulation, FSH not only enhanced overall protein synthesis in a mammalian target of rapamycin-dependent manner but also increased the recruitment of mRNA to polysomes. m7GTP pull-down experiments revealed the functional recruitment of mammalian target of rapamycin and p70 S6 kinase to the 5'cap, further supported by the enhanced phosphorylation of one of p70 S6 kinase targets, the eukaryotic initiation factor 4B. Importantly, the scaffolding eukaryotic initiation factor 4G was also recruited, whereas eukaryotic initiation factor 4E-binding protein, the eukaryotic initiation factor 4E generic inhibitor, appeared to play a minor role in translational regulations induced by FSH, in contrast to what is generally observed in response to anabolic factors. This particular regulation of the translational machinery by FSH stimulation might support mRNA-selective translation, as shown here by quantitative RT-PCR amplification of the c-fos and vascular endothelial growth factor mRNA but not of all FSH target mRNA, in polysomal fractions. These findings add a new level of complexity to FSH biological roles in its natural target cells, which has been underappreciated so far. (Molecular Endocrinology 26: 669-680, 2012)
引用
收藏
页码:669 / 680
页数:12
相关论文
共 46 条
  • [1] Proliferative Activity In Vitro and DNA Repair Indicate that Adult Mouse and Human Sertoli Cells Are Not Terminally Differentiated, Quiescent Cells
    Ahmed, Emad A.
    Barten-van Rijbroek, Angelique D.
    Kal, Henk B.
    Sadri-Ardekani, Hooman
    Mizrak, S. Canan
    van Pelt, Ans M. M.
    de Rooij, Dirk G.
    [J]. BIOLOGY OF REPRODUCTION, 2009, 80 (06) : 1084 - 1091
  • [2] Follicle-stimulating hormone activation of hypoxia-inducible factor-1 by the phosphatidylinositol 3-kinase/AKT/Ras homolog enriched in brain (Rheb)/mammalian target of rapamycin (mTOR) pathway is necessary for induction of select protein markers of follicular differentiation
    Alam, H
    Maizels, ET
    Park, Y
    Ghaey, S
    Feiger, ZJ
    Chandel, NS
    Hunzicker-Dunn, M
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (19) : 19431 - 19440
  • [3] Role of the Phosphatidylinositol-3-Kinase and Extracellular Regulated Kinase Pathways in the Induction of Hypoxia-Inducible Factor (HIF)-1 Activity and the HIF-1 Target Vascular Endothelial Growth Factor in Ovarian Granulosa Cells in Response to Follicle-Stimulating Hormone
    Alam, Hena
    Weck, Jennifer
    Maizels, Evelyn
    Park, Youngkyu
    Lee, Eun Jig
    Ashcroft, Margaret
    Hunzicker-Dunn, Mary
    [J]. ENDOCRINOLOGY, 2009, 150 (02) : 915 - 928
  • [4] New Hierarchical Phosphorylation Pathway of the Translational Repressor eIF4E-binding Protein 1 (4E-BP1) in Ischemia-Reperfusion Stress
    Ayuso, Maria I.
    Hernandez-Jimenez, Macarena
    Martin, Maria E.
    Salinas, Matilde
    Alcazar, Alberto
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (45) : 34355 - 34363
  • [5] Translational control of localized mRNAs: restricting protein synthesis in space and time
    Besse, Florence
    Ephrussi, Anne
    [J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2008, 9 (12) : 971 - 980
  • [6] Phosphorylation of the translational repressor PHAS-I by the mammalian target of rapamycin
    Brunn, GJ
    Hudson, CC
    Sekulic, A
    Williams, JM
    Hosoi, H
    Houghton, PJ
    Lawrence, JC
    Abraham, RT
    [J]. SCIENCE, 1997, 277 (5322) : 99 - 101
  • [7] COBB MH, 1986, J BIOL CHEM, V261, P2994
  • [8] The ERK-dependent signalling is stage-specifically modulated by FSH, during primary Sertoli cell maturation
    Crépieux, P
    Marion, S
    Martinat, N
    Fafeur, V
    Le Vern, Y
    Kerboeuf, D
    Guillou, F
    Reiter, E
    [J]. ONCOGENE, 2001, 20 (34) : 4696 - 4709
  • [9] HORMONAL-REGULATION OF PROTEIN-SYNTHESIS, SECRETION, AND PHOSPHORYLATION IN CULTURED RAT SERTOLI CELLS
    DEPHILIP, RM
    KIERSZENBAUM, AL
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (21): : 6551 - 6555
  • [10] β-arrestin-mediated signaling regulates protein synthesis
    Dewire, Scott M.
    Kim, Jihee
    Whalen, Erin J.
    Ahn, Seungkirl
    Chen, Minyong
    Lefkowitz, Robert J.
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (16) : 10611 - 10620