Induction of Phenol Hydroxylase from Aspergillus niger and Its Optimization

被引:1
|
作者
Bilgi, Mesut [1 ]
Peksel, Aysegul [1 ]
机构
[1] Yildiz Tech Univ, Dept Chem, Davutpasa Campus, TR-34220 Istanbul, Turkey
来源
CHEMISTRYSELECT | 2022年 / 7卷 / 14期
关键词
Aspergillus niger; biocatalysis; enzymes; phenol hydroxylase; ADENINE-DINUCLEOTIDE; DEGRADATION; YEAST; BIOTRANSFORMATION; BIODEGRADATION; IDENTIFICATION; ANIONS; ENZYME;
D O I
10.1002/slct.202103838
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Phenol is a harmful substance that can be present in many industrial wastewaters. Chemical phenol removal is feasible, but the procedure is challenging and expensive. Biological phenol removal is possible under mild conditions with enzymes. Phenol hydroxylase is the main enzyme for phenol removal. In this study, phenol hydroxylase was induced from filamentous fungus Aspergillus niger. The results have shown that it is possible to use Aspergillus niger as a phenol hydroxylase source, but the enzyme has activity in limited temperature and pH conditions. The optimal working conditions of the enzyme are 30 degrees C and pH 6.5. The enzyme has moderate storage and thermal stability, and various compounds inhibit it.
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页数:6
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