microRNA-34a regulates neurite outgrowth, spinal morphology, and function

被引：158

作者：

Agostini, Massimiliano ^{[1
]}

Tucci, Paola ^{[1
,2
]}

Steinert, Joern R. ^{[1
]}

Shalom-Feuerstein, Ruby ^{[3
,4
]}

Rouleau, Matthieu ^{[3
,4
]}

Aberdam, Daniel ^{[3
,4
]}

Forsythe, Ian D. ^{[1
]}

Young, Kenneth W. ^{[1
]}

Ventura, Andrea ^{[5
]}

Concepcion, Carla P. ^{[5
]}

Han, Yoon-Chi ^{[5
]}

Candi, Eleonora ^{[2
]}

Knight, Richard A. ^{[1
]}

Mak, Tak W. ^{[6
]}

Melino, Gerry ^{[1
,2
]}

机构：

[1] Univ Leicester, MRC, Toxicol Unit, Leicester LE1 9HN, Leics, England

[2] Univ Roma Tor Vergata, Biochem Lab, Ist Dermopat Immacolata, Ist Ricovero & Cura Carattere Sci, I-00133 Rome, Italy

[3] Univ Nice Sophia Antipolis, INSERM, U898, F-06107 Nice, France

[4] Technion Israel Inst Technol, INSERTECH, Rappaport Family Inst Res Med Sci, IL-31096 Haifa, Israel

[5] Mem Sloan Kettering Canc Ctr, Canc Genet & Biol Program, New York, NY 10065 USA

[6] Princess Margaret Hosp, Campbell Family Inst Breast Canc Res, Toronto, ON M5G 2C1, Canada

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2011年 / 108卷 / 52期

基金：

英国医学研究理事会;

关键词：

cell death; synaptogenesis; neuronal differentiation; hippocampus; EMBRYONIC STEM-CELLS; GENE-EXPRESSION; P53; DIFFERENTIATION; APOPTOSIS; MIR-34A; P73; ACTIVATION; VARIANTS; NANOG;

D O I：

10.1073/pnas.1112063108

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The p53 family member TAp73 is a transcription factor that plays a key role in many biological processes, including neuronal development. In particular, we have shown that p73 drives the expression of miR-34a, but not miR-34b and c, in mouse cortical neurons. miR-34a in turn modulates the expression of synaptic targets including synaptotagmin-1 and syntaxin-1A. Here we show that this axis is retained in mouse ES cells committed to differentiate toward a neurological phenotype. Moreover, overexpression of miR-34a alters hippocampal spinal morphology, and results in electrophysiological changes consistent with a reduction in spinal function. Therefore, the TAp73/miR-34a axis has functional relevance in primary neurons. These data reinforce a role for miR-34a in neuronal development.

引用

页码：21099 / 21104

页数：6

共 29 条

[1]

Agostini M, 2011, P NATL ACAD SCI US

[2] p53-mediated activation of miRNA34 candidate tumor-suppressor genes [J].

Bommer, Guido T. ;

Gerin, Isabelle ;

Feng, Ying ;

Kaczorowski, Andrew J. ;

Kuick, Rork ;

Love, Robert E. ;

Zhai, Yali ;

Giordano, Thomas J. ;

Qin, Zhaohui S. ;

Moore, Bethany B. ;

MacDougald, Ormond A. ;

Cho, Kathleen R. ;

Fearon, Eric R. .

CURRENT BIOLOGY, 2007, 17 (15) :1298-1307

[3] Transactivation of miR-34a by p53 broadly influences gene expression and promotes apoptosis [J].

Chang, Tsung-Cheng ;

Wentzel, Erik A. ;

Kent, Oliver A. ;

Ramachandran, Kalyani ;

Mullendore, Michael ;

Lee, Kwang Hyuck ;

Feldmann, Georg ;

Yamakuchi, Munekazu ;

Ferlito, Marcella ;

Lowenstein, Charles J. ;

Arking, Dan E. ;

Beer, Michael A. ;

Maitra, Anirban ;

Mendell, Joshua T. .

MOLECULAR CELL, 2007, 26 (05) :745-752

[4] miR-34 miRNAs provide a barrier for somatic cell reprogramming [J].

Choi, Yong Jin ;

Lin, Chao-Po ;

Ho, Jaclyn J. ;

He, Xingyue ;

Okada, Nobuhiro ;

Bu, Pengcheng ;

Zhong, Yingchao ;

Kim, Sang Yong ;

Bennett, Margaux J. ;

Chen, Caifu ;

Ozturk, Arzu ;

Hicks, Geoffrey G. ;

Hannon, Greg J. ;

He, Lin .

NATURE CELL BIOLOGY, 2011, 13 (11) :1353-U154

[5] Additional complexity in p73:: induction by mitogens in lymphoid cells and identification of two new splicing variants ε and ζ [J].

De Laurenzi, V ;

Catani, MV ;

Terrinoni, A ;

Corazzari, M ;

Melino, G ;

Costanzo, A ;

Levrero, M ;

Knight, RA .

CELL DEATH AND DIFFERENTIATION, 1999, 6 (05) :389-390

[6] Two new p73 splice variants, γ and δ, with different transcriptional activity [J].

De Laurenzi, V ;

Costanzo, A ;

Barcaroli, D ;

Terrinoni, A ;

Falco, M ;

Annicchiarico-Petruzzeli, M ;

Levrero, M ;

Melino, G .

JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 188 (09) :1763-1768

[7] Mef2-mediated transcription of the miR379-410 cluster regulates activity-dependent dendritogenesis by fine-tuning Pumilio2 protein levels [J].

Fiore, Roberto ;

Khudayberdiev, Sharof ;

Christensen, Mette ;

Siegel, Gabriele ;

Flavell, Steven W. ;

Kim, Tae-Kyung ;

Greenberg, Michael E. ;

Schratt, Gerhard .

EMBO JOURNAL, 2009, 28 (06) :697-710

[8] BMP-4 induces a Smad-dependent apoptotic cell death of mouse embryonic stem cell-derived neural precursors [J].

Gambaro, K. ;

Aberdam, E. ;

Virolle, T. ;

Aberdam, D. ;

Rouleau, M. .

CELL DEATH AND DIFFERENTIATION, 2006, 13 (07) :1075-1087

[9] A microRNA component of the p53 tumour suppressor network [J].

He, Lin ;

He, Xingyue ;

Lim, Lee P. ;

De Stanchina, Elisa ;

Xuan, Zhenyu ;

Liang, Yu ;

Xue, Wen ;

Zender, Lars ;

Magnus, Jill ;

Ridzon, Dana ;

Jackson, Aimee L. ;

Linsley, Peter S. ;

Chen, Caifu ;

Lowe, Scott W. ;

Cleary, Michele A. ;

Hannon, Gregory J. .

NATURE, 2007, 447 (7148) :1130-U16

[10] The miR-34 family in cancer and apoptosis [J].

Hermeking, H. .

CELL DEATH AND DIFFERENTIATION, 2010, 17 (02) :193-199

← 1 2 3 →