Stereological Evaluation of Rabbit Fetus Liver after Xenotransplantation of Human Wharton's Jelly-Derived Mesenchymal Stromal Cells

被引：0

作者：

Khodabandeh, Z. ^{[1
]}

Rezaeian, L. ^{[2
]}

Edalatmanesh, M. A. ^{[3
]}

Mogheiseh, A. ^{[4
]}

Tanideh, N. ^{[5
]}

Dianatpour, M. ^{[6
]}

Zare, Sh. ^{[1
]}

Bordbar, H. ^{[7
,8
]}

Baghban, N. ^{[8
]}

Tamadon, A. ^{[9
]}

机构：

[1] Shiraz Univ Med Sci, Stem Cells Technol Res Ctr, Shiraz, Iran

[2] Farhangian Univ, Dept Biol, Fac Sci, Tehran, Iran

[3] Islamic Azad Univ, Shiraz Branch, Dept Biol, Fac Sci, Shiraz, Iran

[4] Shiraz Univ, Sch Vet Med, Dept Clin Sci, Shiraz, Iran

[5] Shiraz Univ Med Sci, Sch Med, Dept Pharmacol, Shiraz, Iran

[6] Shiraz Univ Med Sci, Sch Med, Dept Human Genet, Shiraz, Iran

[7] Shiraz Univ Med Sci, Histomorphometry & Stereol Res Ctr, Shiraz, Iran

[8] Shiraz Univ Med Sci, Dept Anat, Shiraz, Iran

[9] PerciaVista R&D Co, Shiraz 7514633196, Iran

来源：

INTERNATIONAL JOURNAL OF ORGAN TRANSPLANTATION MEDICINE | 2022年 / 13卷 / 01期

关键词：

Xenotransplantation; Mesenchymal stromal/stem cells; Wharton's jelly; Stereology; Liver; STEM-CELLS; INTRAUTERINE XENOTRANSPLANTATION; ANIMAL-MODELS; TRANSPLANTATION; ENGRAFTMENT; FIBROSIS; CORD;

D O I：

暂无

中图分类号：

R3 [基础医学]; R4 [临床医学];

学科分类号：

1001 ; 1002 ; 100602 ;

摘要：

Background: In-utero xenotransplantation of stem cells in abnormal fetuses effectively treats several genetic illnesses. Objective: The current research aimed to evaluate structural and morphological alterations in the liver of rabbit fetuses following xenotransplantation of human Wharton's jelly-derived mesenchymal stromal cells (hWJ-MSCs), using a stereological technique. Methods: All hWJ-MSCs were isolated from the human umbilical cord, and their authenticity was established by flow-cytometry and differentiation. At gestational day 14, the rabbits were anesthetized, and hWJ-MSCs were injected into the uteri of 24 fetuses. Twenty-two fetuses were born successfully. Ten rabbit liver specimens were prepared from injected fetuses including; eight rabbits on day three following birth and two rabbits on the 21st post-natal day. The non-injected fetuses were considered positive controls. The livers of the control and hWJ-MSCs-treated rabbits were fixed, processed, stained, and examined through stereological approaches. Results: In the hWJ-MSCs-treated group, the mean liver weight and volume increased by 42% and 78% compared to the control group. The total volume of the hepatocytes increased by 63%, and that of sinusoids by three folds in the treated rabbits. The total volume of the central veins increased by 70%. The total number corresponding to hepatocytes in the experimental group increased by 112% compared to the rabbits in the control. The total volume of the hepatocyte nuclei in the experimental group increased by 117% compared to the rabbits in the control. Conclusion:After xenotransplantation of human MSCs, host tissue microenvironments (here, the rabbit liver) were altered and these included quantitative factors corresponding to the liver tissue and hepatocyte morphometric indices.

引用

页码：15 / 24

页数：10

共 41 条

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