Epitope mapping of antibodies to VlsE protein of Borrelia burgdorferi in post-Lyme disease syndrome

被引：25

作者：

Chandra, Abhishek ^{[1
]}

Latov, Norman ^{[1
]}

Wormser, Gary P. ^{[2
]}

Marques, Adriana R. ^{[3
]}

Alaedini, Armin ^{[1
]}

机构：

[1] Cornell Univ, Weill Cornell Med Coll, Dept Neurol & Neurosci, New York, NY 10021 USA

[2] New York Med Coll, Dept Med, Div Infect Dis, Valhalla, NY 10595 USA

[3] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA

来源：

CLINICAL IMMUNOLOGY | 2011年 / 141卷 / 01期

基金：

美国国家卫生研究院;

关键词：

Lyme disease; Post-Lyme disease syndrome; Chronic Lyme disease; VlsE; Epitope mapping; Antibody; IMMUNODOMINANT CONSERVED REGION; VARIABLE SURFACE-ANTIGEN; ANTIBIOTIC-TREATMENT; PERSISTENT SYMPTOMS; DIAGNOSIS; HISTORY; PEPTIDE; DOMAIN;

D O I：

10.1016/j.clim.2011.06.005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The VlsE lipoprotein of Borrelia burgdorferi elicits a strong immune response during the course of Lyme disease. The present study was aimed at characterization of the epitopes of VlsE targeted by the antibody response in patients with post-Lyme disease syndrome, a condition characterized by persisting symptoms of pain, fatigue, and/or neurocognitive impairment despite antibiotic treatment of B. burgdorferi infection. Epitope mapping was carried out using microarrays that contained synthesized overlapping peptides covering the full sequence of VlsE from B. burgdorferi B31. In addition to the previously characterized IR6 region in the variable domain, specific sequences in the N- and C-terminal invariable domains of VlsE were found to be major B cell epitopes in affected patients. The crystal structure of VlsE indicated that the newly described epitopes form a contiguous region in the surface-exposed membrane-proximal part of the monomeric form of the protein. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：103 / 110

页数：8

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