Phenotypic and phylogenetic characterization of native peanut Bradyrhizobium isolates obtained from Cordoba, Argentina

被引:34
作者
Munoz, Vanina [1 ]
Ibanez, Fernando [1 ]
Laura Tonelli, Maria [1 ]
Valetti, Lucio [1 ]
Soledad Anzuay, Maria [1 ]
Fabra, Adriana [1 ]
机构
[1] Univ Nacl Rio Cuarto, Dept Ciencias Nat, Fac Ciencias Exactas Fis Quim & Nat, RA-5800 Cordoba, Argentina
关键词
Arachis hypogaea L; Bradyrhizobium; Phylogeny; Core and nodulation genes; B; yuanmingense; iriomotense; HORIZONTAL GENE-TRANSFER; SP-NOV; BLASTOBACTER-DENITRIFICANS; MOLECULAR DIVERSITY; ARACHIS-HYPOGAEA; NODULATION GENES; RIBOSOMAL-RNA; RHIZOBIA; NODULES; LEGUME;
D O I
10.1016/j.syapm.2011.04.007
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Peanut is an economically important legume nodulated by slow-growing bacteria of the genus Bradyrhizobium. In this study, a collection of native slow-growing peanut rhizobial isolates from Argentina was obtained and characterized. The phenotypical characterization included the determination of the symbiotic properties, whereas the genetic and phylogenetic diversity was assessed through ERIC-PCR and sequencing of the internal transcribed spacer (ITS) region, as well as the dnaK and nodA genes. The results obtained indicated that peanut nodulating bradyrhizobia were phenotypically and genotypically diverse, and included locally adapted variants of B. yuanmingense and B. iriomotense carrying novel nodA alleles. (C) 2011 Elsevier GmbH. All rights reserved.
引用
收藏
页码:446 / 452
页数:7
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