Crystallization and preliminary X-ray crystallographic studies of glutamate racemase from Lactobacillus fermenti

被引：2
|
作者：
Lee, KS
Park, SM
Hwang, KY [1 ]
Chi, YM
机构：
[1] Korea Univ, Div Biotechnol & Genet Engn, Seoul 136701, South Korea
[2] Korea Inst Sci & Technol, Div Life Sci, Biomed Res Ctr, Seoul 136791, South Korea
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2005年 / 61卷
关键词：
D O I：
10.1107/S1744309104034426
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Glutamate racemase catalyzes the conversion of L-glutamic acid to D-glutamic acid and vice versa. Since D-glutamic acid is one of the essential amino acids present in peptidoglycan, glutamate racemase has been considered to be an attractive target for the design of new antibacterial drugs. Glutamate racemase from Lactobacillus fermenti has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 8000 as a precipitant. The crystals belong to the orthorhombic space group C222(1), with unit-cell parameters a = 98.32, b = 184.09, c = 45.99 angstrom. The asymmetric unit contains one molecule, corresponding to a V-M value of 1.84 angstrom(3) Da(-1). A complete data set has been collected from the native enzyme at 2.28 angstrom resolution using a synchrotron-radiation source.
引用
收藏
页码：199 / 201
页数：3
相关论文
共 50 条
  • [31] Crystallization and preliminary X-ray crystallographic studies of dipeptidyl peptidase 11 from Porphyromonas gingivalis
    Sakamoto, Yasumitsu
    Suzuki, Yoshiyuki
    Iizuka, Ippei
    Tateoka, Chika
    Roppongi, Saori
    Fujimoto, Mayu
    Gouda, Hiroaki
    Nonaka, Takamasa
    Ogasawara, Wataru
    Tanaka, Nobutada
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2015, 71 : 206 - 210
  • [32] Crystallization and preliminary X-ray crystallographic studies of the alkanesulfonate FMN reductase from Escherichia coli
    Gao, BL
    Bertrand, A
    Boles, WH
    Ellis, HR
    Mallett, TC
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2005, 61 : 837 - 840
  • [33] Protein expression, crystallization and preliminary X-ray crystallographic studies of LidA from Legionella pneumophila
    Zhu, Wenzhuang
    Meng, Geng
    Liu, Yong
    Zhang, Feiyun
    Zheng, Xiaofeng
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 637 - 639
  • [34] Crystallization and preliminary X-ray crystallographic studies on 4-α-glucanotransferase from Thermotoga maritima
    Roujeinikova, A
    Raasch, C
    Sedelnikova, S
    Liebl, W
    Rice, DW
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2001, 57 : 1046 - 1047
  • [35] Crystallization and preliminary X-ray crystallographic studies on recombinant human carnitine acetyltransferase
    Lian, W
    Govindasamy, L
    Gu, YR
    Kukar, T
    Agbandje-McKenna, M
    McKenna, R
    Wu, DH
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2002, 58 : 1193 - 1194
  • [36] Crystallization and preliminary X-ray crystallographic studies of HsIU mutant in Escherichia coli
    Song, JJ
    Lee, CS
    Kim, YS
    Kim, JJ
    Im, YJ
    Kim, HH
    Yoo, SJ
    Seong, IS
    Chung, CH
    Eom, SH
    BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2000, 21 (07) : 747 - 748
  • [37] Protein expression, crystallization and preliminary X-ray crystallographic studies of YJbK from Bacillus subtilis
    Dai, XY
    Liu, Y
    Liang, YH
    Zheng, XF
    Luo, M
    Li, LF
    Su, XD
    PROTEIN AND PEPTIDE LETTERS, 2005, 12 (07): : 663 - 664
  • [38] Crystallization and preliminary X-ray crystallographic studies of Escherichia coli xanthine phosphoribosyltransferase
    Vos, S
    deJersey, J
    Martin, JL
    JOURNAL OF STRUCTURAL BIOLOGY, 1996, 116 (02) : 330 - 334
  • [39] Crystallization and preliminary X-ray crystallographic studies of pig heart carbonyl reductase
    Aoki, Ken-ichi
    Tanaka, Nobutada
    Ishikura, Shuhei
    Araki, Naoko
    Imamura, Yorishige
    Hara, Akira
    Nakamura, Kazuo T.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2006, 62 : 1037 - 1040
  • [40] Protein expression, crystallization and preliminary X-ray crystallographic studies on HSCARG from Homo sapiens
    Dai, Xueyu
    Gu, Xiaocheng
    Luo, Ming
    Zheng, Xiaofeng
    PROTEIN AND PEPTIDE LETTERS, 2006, 13 (09): : 955 - 957
12345