Heat Induces Oxidative Stress: Reproductive Organ Weights and Serum Metabolite Profile, Testes Structure, and Function Impairment in Male Cavy (Cavia porcellus)

被引：17

作者：

Ngoula, Ferdinand ^{[1
]}

Lontio, Fulbert Aime ^{[1
]}

Tchoffo, Herve ^{[1
]}

Tsague, Faustin Pascal Manfo ^{[2
]}

Djeunang, Romeo-Marcial ^{[1
]}

Vemo, Bertin Narcisse ^{[1
]}

Moffo, Frederic ^{[1
]}

Motchewo, Nadege Djuissi ^{[1
]}

机构：

[1] Univ Dschang, Fac Agron & Agr Sci, Anim Physiol & Hlth Res Unit, Dschang, Cameroon

[2] Univ Buea, Fac Sci, Dept Biochem & Mol Biol, Buea, Cameroon

来源：

FRONTIERS IN VETERINARY SCIENCE | 2020年 / 7卷

关键词：

heat stress; male guinea pig; oxidative stress; reproduction; heat shock proteins; TEMPERATURE; SEMEN;

D O I：

10.3389/fvets.2020.00037

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The present study was designed to evaluate the effects of heat that induces oxidative stress on reproduction organ weight and serum biochemical, testes structure, and function in male guinea pig (Cavia porcellus). Forty-eight male guinea pigs with an average weight of 330.56 +/- 23.62 g, aged 3-4 months, were distributed into four groups of 12 animals each. One group (control) was maintained to ambient temperature (20-25 degrees C), while other groups (Groups 2-4) were exposed daily for 6 h, to 32 +/- 1 degrees C, 39 +/- 1 degrees C, and 46 +/- 1 degrees C, respectively. All animals were sacrificed after 60 days' exposure and their reproductive characteristics values were determined. Results revealed a significant decrease (p < 0.05) of the weight of testes, epididymis, vas deferens, and accessory glands in cavies exposed to the highest temperature investigated (46 +/- 1 degrees C), compared to the control animals. There was a significant (p < 0.05) reduction of serum testosterone and LH levels in all heat stress-exposed groups (>= 46 +/- 1 degrees C) when compared to the control group. Heat stress significantly (p < 0.05) decreased sperm mobility, sperm count, and testicular antioxidant enzymes, while increasing testicular malondialdehyde content. However, the serum level of HSP-40 increased in the animals exposed to 39 +/- 1 degrees C and decreased when the cavies were exposed to 46 +/- 1 degrees C. In conclusion, exposure to heat-induced oxidative stress results in impairment of reproduction organ weight and serum biochemical, testes structure, and function in male cavies.

引用

页数：7