An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis

被引：22

作者：

Higa, Yumiko ^{[1
]}

Uemura, Ryoko ^{[1
,2
]}

Yamazaki, Wataru ^{[1
,2
]}

Goto, Shinya ^{[1
]}

Goto, Yoshitaka ^{[1
,2
]}

Sueyoshi, Masuo ^{[1
,2
]}

机构：

[1] Miyazaki Univ, Fac Agr, Dept Vet Med Sci, 1-1 Gakuenkibanadai Nishi, Miyazaki 8892192, Japan

[2] Miyazaki Univ, Ctr Anim Dis Control, 1-1 Gakuenkibanadai Nishi, Miyazaki 8892192, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2016年 / 78卷 / 08期

关键词：

cattle; loop-mediated isothermal amplification (LAMP) assay; Mycoplasma bovis; respiratory disease; CHAIN-REACTION ASSAY; RAPID DETECTION; PCR; IDENTIFICATION; PNEUMONIA; CATTLE; RNA; PERFORMANCE; AGALACTIAE; DIAGNOSIS;

D O I：

10.1292/jvms.15-0459

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We improved a loop-mediated isothermal amplification (LAMP) assay permitting sensitive and rapid Mycoplasma bovis detection. A total of 55 bacterial strains were examined in this study, including 33 M. bovis strains, 14 non-M bovis mycoplasmas and eight non-mycoplasma bacterial strains. M bovis was successfully detected by the LAMP assay within 60 min without cross-reaction to any other bacteria. Furthermore, a total of 135 nasal swab samples were tested directly using our LAMP assays, the previously reported LAMP assay, conventional PCR assay without pre-culture and comparing standard culture methods. The improved LAMP assay showed sensitivity and specificity of 97.2% and 90.9%, respectively (with a kappa coefficient of 0.8231), and the sensitivity of our revised LAMP assay was increased compared to existing methods.

引用

页码：1343 / 1346

页数：4

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