High performance liquid chromatographic determination of diazinon, permethrin, DEET (N, N-diethyl-m-toluamide), and their metabolites in rat plasma and urine

被引:9
作者
Abu-Qare, AW [1 ]
Abou-Donia, MB [1 ]
机构
[1] Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA
来源
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 2001年 / 370卷 / 04期
关键词
D O I
10.1007/s002160100780
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A rapid method was developed for the analysis of the insecticide (A) diazinon (O,O-diethyl O-2-isopropyl-6-methylpyridimidinyl) phosphorothioate, its metabolites (B) diazoxon (O,O-diethyl O-2-isopropyl-6-methylpyridimidinyl) phosphate, and (C) 2-isopropyl-6-methyl-4-pyrimidinol, the insecticide (D) permethrin [3-(2,2-dichloro-ethenyl)-2,2-dimethylcyclopropanecarboxylic acid (3-phenoxyphenyl)methylester], its metabolites (E) m-phen-oxybenzyl alcohol, and (F) m-phenoxybenzoic acid, the insect repellent (G) DEET (NN-diethyl-m-toluamide), and its metabolites (H) m-toluamide and (I) m-toluic acid in rat plasma and urine. The method is based on using Cls Sep-Pak cartridges (Waters Corporation, Milford, Mass., U.S.A.) for solid phase extraction and high performance liquid chromatography with a reversed phase C-18 column, and absorbance detection at 230 nm for compounds A, B, and C, and at 210 nm for compounds D-I. The compounds were separated using a gradient from 1% to 99% acetonitrile in water (pH 3.0) at a flow rate ranging between 1 and 1.7 mL/min in a period of 17 min. The limits of detection were ranged between 20 and 100 ng/mL, while limits of quantification were 80-200 ng/mL. The relationship between peak areas and concentration was linear over a range of 100-1000 ng/mL. This method was applied to determine the above insecticides and their metabolites following dermal administration in rats.
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收藏
页码:403 / 407
页数:5
相关论文
共 31 条
[1]   Neurotoxicity resulting from coexposure to pyridostigmine bromide, DEET, and permethrin: Implications of Gulf War chemical exposures [J].
AbouDonia, MB ;
Wilmarth, KR ;
Jensen, KF ;
Oehme, FW ;
Kurt, TL .
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH, 1996, 48 (01) :35-56
[2]   LABORATORY AND FIELD EVALUATIONS OF A REPELLENT SOAP CONTAINING DIETHYL TOLUAMIDE (DEET) AND PERMETHRIN AGAINST PHLEBOTOMINE SAND FLIES (DIPTERA, PSYCHODIDAE) IN VALLE DEL CAUCA, COLOMBIA [J].
ALEXANDER, B ;
CADENA, H ;
USMA, MC ;
ROJAS, CA .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1995, 52 (02) :169-173
[3]   TOXICOKINETICS OF PERMETHRIN IN THE RAT [J].
ANADON, A ;
MARTINEZLARRANAGA, MR ;
DIAZ, MJ ;
BRINGAS, P .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 1991, 110 (01) :1-8
[4]   Determination of metabolites of pyrethroids in human urine using solid-phase extraction and gas chromatography mass spectrometry [J].
Angerer, J ;
Ritter, A .
JOURNAL OF CHROMATOGRAPHY B, 1997, 695 (02) :217-226
[5]   Insect repellents: An overview [J].
Brown, M ;
Hebert, AA .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1997, 36 (02) :243-249
[6]  
BURGESS I, 1992, PHARM J, V246, P692
[7]  
ELLIN RI, 1982, J CHROMATOGR, V228, P235
[8]  
EPA, 1998, OFF PEST PROGR, P1
[9]   IDENTIFICATION OF ORGANO-PHOSPHORUS INSECTICIDES AND THEIR HYDROLYSIS PRODUCTS BY LIQUID-CHROMATOGRAPHY IN COMBINATION WITH UV AND THERMOSPRAY MASS SPECTROMETRIC DETECTION [J].
FARRAN, A ;
DEPABLO, J ;
BARCELO, D .
JOURNAL OF CHROMATOGRAPHY, 1988, 455 :163-172
[10]   ANALYSIS OF DIETHYLTOLUAMIDE (DEET) FOLLOWING INTENTIONAL ORAL INGESTION OF MUSCOL(R) [J].
FRASER, AD ;
MACNEIL, A ;
THERIAULT, M ;
MORZYCKI, W .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1995, 19 (03) :197-199