Palmitoyl ceramide promotes milk sphingomyelin gel phase domains formation and affects the mechanical properties of the fluid phase in milk-SM/DOPC supported membranes

被引:14
作者
Murthy, Appala Venkata Ramana [1 ]
Guyomarc'h, Fanny [1 ]
Lopez, Christelle [1 ]
机构
[1] Agrocampus Ouest, INRA, STLO, F-35000 Rennes, France
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2018年 / 1860卷 / 03期
关键词
Ceramide; Milk sphingomyelin; Membrane domain; Supported bilayer; Atomic force microscopy; ATOMIC-FORCE MICROSCOPY; FAT GLOBULE-MEMBRANE; LIPID-BILAYERS; ALKALINE SPHINGOMYELINASE; NANOMECHANICAL PROPERTIES; BIOPHYSICAL PROPERTIES; DETERGENT-RESISTANT; CHOLESTEROL; SPHINGOLIPIDS; PHOSPHATIDYLCHOLINE;
D O I
10.1016/j.bbamem.2017.12.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ceramides are minor structural components of membranes involved in biological functions. In the milk fat globule membrane (MFGM), ceramides are susceptible to affect the lateral packing of polar lipids, especially the milk sphingomyelin (MSM). To investigate this, palmitoylceramide (PCer) was added to MSM/DOPC (dioleoylphosphatidylcholine) in order to form hydrated lipid bilayers. Differential scanning calorimetry evidenced interactions of PCer with the MSM in the solid -ordered phase to form MSM/PCer structures with a higher thermostability than MSM. Atomic force microscopy revealed that PCer modified lipid packing in both the liquid-disordered DOPC phase where it increased thickness and mechanical stability, and the solid -ordered MSM phase where it recruited MSM molecules yet initially in the liquid phase at 26 degrees C and then increased the area of the MSM/PCer domains. The effect of PCer on the mechanical properties of the MSM-rich domains remains to be elucidated. These results bring new insights on the role of ceramides in the control of biophysical and biological properties of the MFGM. They also open perspectives for the design of emulsions and liposomes, using milk polar lipids as food-grade ingredients.
引用
收藏
页码:635 / 644
页数:10
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