Overexpression of ADH1 and HXT1 genes in the yeast Saccharomyces cerevisiae improves the fermentative efficiency during tequila elaboration

被引：11

作者：

Gutierrez-Lomeli, Melesio ^{[1
,2
]}

Torres-Guzman, Juan Carlos ^{[3
]}

Gonzalez-Hernandez, Gloria Angelica ^{[3
]}

Cira-Chavez, Luis Alberto ^{[1
,2
]}

Pelayo-Ortiz, Carlos ^{[2
]}

Ramirez-Cordova, Jose de Jesus ^{[1
]}

机构：

[1] Ctr Invest & Asisten Tecnol & Diseno Estado Jalis, Unidad Biotecnol, Guadalajara 44270, Jalisco, Mexico

[2] Univ Guadalajara, Ctr Univ Ciencias Exactas & Ingn, Guadalajara 44430, Jalisco, Mexico

[3] Univ Guanajuato, Fac Quim, Inst Invest Biol Expt, Guanajuato, Mexico

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2008年 / 93卷 / 04期

关键词：

Saccharomyces cerevisiae; Agave tequilana Weber blue variery; fermentative efficiency; alcohol dehydrogenase; transporter hexose;

D O I：

10.1007/s10482-007-9213-z

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This work assessed the effect of the overexpression of ADH1 and HXT1 genes in the Saccharomyces cerevisiae AR5 strain during fermentation of Agave tequilana Weber blue variety must. Both genes were cloned individually and simultaneously into a yeast centromere plasmid. Two transformant strains overexpressing ADH1 and HXT1 individually and one strain overexpressing both genes were randomly selected and named A1, A3 and A5 respectively. Overexpression effect on growth and ethanol production of the A1, A3 and A5 strains was evaluated in fermentative conditions in A. tequilana Weber blue variety must and YPD medium. During growth in YPD and Agave media, all the recombinant strains showed lower cell mass formation than the wild type AR5 strain. Adh enzymatic activity in the recombinant strains A1 and A5 cultivated in A. tequilana and YPD medium was higher than in the wild type. The overexpression of both genes individually and simultaneously had no significant effect on ethanol formation; however, the fermentative efficiency of the A5 strain increased from 80.33% to 84.57% and 89.40% to 94.29% in YPD and Agave medium respectively.

引用

页码：363 / 371

页数：9