Activin receptor mRNA expression by neurons of the avian ciliary ganglion

Previous studies have suggested that activin may serve as a neurodifferentiation factor regulating somatostatin expression in neurons of the avian ciliary ganglion (CG), As one aspect of examining the role of activin in CG development, we inquired whether any of the known activin receptors are expressed by developing CG neurons in vivo. In addition, we examined whether activin A mRNA is expressed in the choroid layer and iris of the chicken eye. Oligonucleotide primers were designed for the chicken activin receptor type IIA (cActR-IIA), type IIB (cActR-IIB), and activin A. In reverse-transcription - polymerase chain reaction (rtPCR), an appropriately sized product was amplified from CG cDNA using primers to the cActR-IIA but not the cActR-IIB, Sequencing confirmed the identity of the PCR product as a fragment of the cActR-IIA. It thus appears that mRNA for the type IIA but not the type IIB activin receptor is expressed in the chicken CG, An antisense strand digoxigenin-labeled riboprobe complimentary to a 358-bp portion of the cActR-IIA kinase region hybridized to cells within cryostat sections of embryonic CG, From E6.5-E18, hybridization of this probe appears to be specific for cells with a neuronal morphology, Using rtPCR with activin A-specific primers we detected activin mRNA in the choroid layer of E14 and E19 eyes, and from the iris at E14, Our results are consistent with a role for activin as a neurodifferentiation factor in vivo, and imply that within the CG, the cActR-IIA is specifically expressed by neurons, and that activin A is expressed in the targets of these neurons. (C) 1997 John Wiley & Sons, Inc.